2026-10-24_00000419|hard|9XCX

Cryo-EM structure of HalM2, a class II lanthipeptide synthetase, bound with substrate and ADP; Electron microscopy 3.73Å
Protein #1 990 residues (97% resolved)

MKTPLTSEHPSVPTTLPHTNDTDWLEQLHDILSIPVTEEIQKYFHAENDLFSFFYTPFLQFTYQSMSDYFMTFKTDMALIERQSLLQSTLTAVHHRLFHLTHRTLISEMHIDKLTVGLNGSTPHERYMDFNHKFNKTSKSKNLFNIYPILGKLVVNETLRTINFVKKIIQHYMKDYLLLSDFFKEKDLRLTNLQLGVGDTHVNGQCVTILTFASGQKVVYKPRSLSIDKQFGEFIEWVNSKGFQPSLRIPIAIDRQTYGWYEFIPHQEATSEDEIERYYSRIGGYLAIAYLFGATDLHLDNLIACGEHPMLIDLETLFTNDLDCYDSAFPFPALARELTQSVFGTLMLPITIASGKLLDIDLSAVGGGKGVQSEKIKTWVIVNQKTDEMKLVEQPYVTESSQNKPTVNGKEANIGNYIPHVTDGFRKMYRLFLNEIDELMDHNGPIFAFESCQIRHVFRATHVYAKFLEASTHPDYLQEPTRRNKLFESFWNITSLMAPFKKIVPHEIAELENHDIPYFVLTCGGTIVKDGYGRDIADLFQSSCIERVTHRLQQLGSEDEARQIRYIKSSLATLTNGDWTPSHEKTPMSPASADREDGYFLREAQAIGDDILAQLIWEDDRHAAYLIGVSVGMNEAVTVSPLTPGIYDGTLGIVLFFDQLAQQTGETHYRHAADALLEGMFKQLKPELMPSSAYFGLGSLFYGLMVLGLQRSDSHIIQKAYEYLKHLEECVQHEETPDFVSGLSGVLYMLTKIYQLTNEPRVFEVAKTTASRLSVLLDSKQPDTVLTGLSHGAAGFALALLTYGTAANDEQLLKQGHSYLVYERNRFNKQENNWVDLRKGNAYQTFWCHGAPGIGISRLLLAQFYDDELLHEELNAALNKTISDGFGHNHSLCHGDFGNLDLLLLYAQYTNNPEPKELARKLAISSIDQAHTYGWKLGLNHSDQLQGMMLGVTGIGYQLLRHINPTVPSILALELPSSTLTEKELRIHDR

MKTPLTSEHPSVPTTLPHTNDTDWLEQLHDILSIPVTEEIQKYFHAENDLFSFFYTPFLQFTYQSMSDYFMTFKTDMALIERQSLLQSTLTAVHHRLFHLTHRTLISEMHIDKLTVGLNGSTPHERYMDFNHKFNKTSKSKNLFNIYPILGKLVVNETLRTINFVKKIIQHYMKDYLLLSDFFKEKDLRLTNLQLGVGDTHVNGQCVTILTFASGQKVVYKPRSLSIDKQFGEFIEWVNSKGFQPSLRIPIAIDRQTYGWYEFIPHQEATSEDEIERYYSRIGGYLAIAYLFGATDLHLDNLIACGEHPMLIDLETLFTNDLDCYDSAFPFPALARELTQSVFGTLMLPITIASGKLLDIDLSAVGGGKGVQSEKIKTWVIVNQKTDEMKLVEQPYVTESSQNKPTVNGKEANIGNYIPHVTDGFRKMYRLFLNEIDELMDHNGPIFAFESCQIRHVFRATHVYAKFLEASTHPDYLQEPTRRNKLFESFWNITSLMAPFKKIVPHEIAELENHDIPYFVLTCGGTIVKDGYGRDIADLFQSSCIERVTHRLQQLGSEDEARQIRYIKSSLATLTNGDWTPSHEKTPMSPASADREDGYFLREAQAIGDDILAQLIWEDDRHAAYLIGVSVGMNEAVTVSPLTPGIYDGTLGIVLFFDQLAQQTGETHYRHAADALLEGMFKQLKPELMPSSAYFGLGSLFYGLMVLGLQRSDSHIIQKAYEYLKHLEECVQHEETPDFVSGLSGVLYMLTKIYQLTNEPRVFEVAKTTASRLSVLLDSKQPDTVLTGLSHGAAGFALALLTYGTAANDEQLLKQGHSYLVYERNRFNKQENNWVDLRKGNAYQTFWCHGAPGIGISRLLLAQFYDDELLHEELNAALNKTISDGFGHNHSLCHGDFGNLDLLLLYAQYTNNPEPKELARKLAISSIDQAHTYGWKLGLNHSDQLQGMMLGVTGIGYQLLRHINPTVPSILALELPSSTLTEKELRIHDR
Protein #2 65 residues (54% resolved)

MVNSKDLRNPEFRKAQGLQFVDEVNEKELSSLAGSGDVHAQTTWPCATVGVSVALCPTTKCTSQC

MVNSKDLRNPEFRKAQGLQFVDEVNEKELSSLAGSGDVHAQTTWPCATVGVSVALCPTTKCTSQC
Non polymer #3 ADP

InChI=1S/C10H15N5O10P2/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(24-10)1-23-27(21,22)25-26(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1

c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@](=O)(O)OP(=O)(O)O)O)O)N
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Reference