2026-10-24_00000416|hard|9XCU
Cryo-EM structure of HalM1 (C963AH964A mutant), a class II lanthipeptide synthetase, bound with substrate; Electron microscopy 3.81Å
Protein #1 1059 residues (94% resolved)
MRELQNALYFSEVVFGPNLEKIVGEKRLNFWLKLIGEDPENLKEFLSRKGNSFEEQTLPEKEAIVPNRLGEEALEKVREELEFLNTYSTKHVRRVKELGVQIPFEGILLPFISMYIEKFQQQQLRKKIGPIHEEIWTQIVQDITSKLNAILHRTLILELNVARVTSQLKGDTPEERFAYYSKTYLGKREVTHRLYSEYPVVLRLLFTTISHHISFITEILERVANDREAIETEFSPCSPIGTLASLHLNSGDAHHKQRTVTILEFSSSLKLVYKPRSLKVDGVFNGLLAFLNDRTGEVIKDQYCPKVLQRDGYGYVEFVTHQSCQSLEEVSDFYERLGSLMSLSYVLNSSDFHFENIIAHGPYPVLIDLETIIHNTADSSEETSTAMDRAFRMLNDSVLSTGMLPSSIYYRDQPNMKGLNVGGVSKSEGQKTPFKVNQIANRNTDEMRIEKDHVTLSSQKNLPIFQSAAMESVHFLDQIQKGFTSMYQWIEKNKQEFKEQVRKFEGVPVRAVLRSTTRYTELLKSSYHPDLLRSALDREVLLNRLTVDSVMTPYLKEIIPLEVEDLLNGDVPYFYTLPEERALYQEASAINSTFFTTSIFHKIDQKIDKLGIEDHTQQMKILHMSMLASNANHYADVADLDIQKGHTIKNEQYVEMAKDIGDYLMELSVEGENQGEPDLCWISTVLEGSSEIIWDISPVGEDLYNGSAGVALFYAYLFKITGEKRYQEIAYKALVPVRRSVAQFQHHPNWSIGAFNGASGYLYAMGTIAALFNDERLKHEVTRSIPHIEPMIHEDKIYDFIGGSAGALKVFLSLSGLFDEPKFLELAIACSEHLMKNAIKTDQGIGWKPPWEVTPLTGFSHGVSGVMASFIELYQQTGDERLLSYIDQSLAYERSFFSEQEENWLTPNKETPVVAWCHGAPGILVSRLLLKKCGYLDEKVEKEIEVALSTTIRKGLGNNRSLCHGDFGQLEILRFAAEVLGDSYLQEVVNNLSGELYNLFKTEGYQSGTSRGTESVGLMVGLSGFGYGLLSAAYPSAVPSILTLDGEIQKYREPHEANH
MRELQNALYFSEVVFGPNLEKIVGEKRLNFWLKLIGEDPENLKEFLSRKGNSFEEQTLPEKEAIVPNRLGEEALEKVREELEFLNTYSTKHVRRVKELGVQIPFEGILLPFISMYIEKFQQQQLRKKIGPIHEEIWTQIVQDITSKLNAILHRTLILELNVARVTSQLKGDTPEERFAYYSKTYLGKREVTHRLYSEYPVVLRLLFTTISHHISFITEILERVANDREAIETEFSPCSPIGTLASLHLNSGDAHHKQRTVTILEFSSSLKLVYKPRSLKVDGVFNGLLAFLNDRTGEVIKDQYCPKVLQRDGYGYVEFVTHQSCQSLEEVSDFYERLGSLMSLSYVLNSSDFHFENIIAHGPYPVLIDLETIIHNTADSSEETSTAMDRAFRMLNDSVLSTGMLPSSIYYRDQPNMKGLNVGGVSKSEGQKTPFKVNQIANRNTDEMRIEKDHVTLSSQKNLPIFQSAAMESVHFLDQIQKGFTSMYQWIEKNKQEFKEQVRKFEGVPVRAVLRSTTRYTELLKSSYHPDLLRSALDREVLLNRLTVDSVMTPYLKEIIPLEVEDLLNGDVPYFYTLPEERALYQEASAINSTFFTTSIFHKIDQKIDKLGIEDHTQQMKILHMSMLASNANHYADVADLDIQKGHTIKNEQYVEMAKDIGDYLMELSVEGENQGEPDLCWISTVLEGSSEIIWDISPVGEDLYNGSAGVALFYAYLFKITGEKRYQEIAYKALVPVRRSVAQFQHHPNWSIGAFNGASGYLYAMGTIAALFNDERLKHEVTRSIPHIEPMIHEDKIYDFIGGSAGALKVFLSLSGLFDEPKFLELAIACSEHLMKNAIKTDQGIGWKPPWEVTPLTGFSHGVSGVMASFIELYQQTGDERLLSYIDQSLAYERSFFSEQEENWLTPNKETPVVAWCHGAPGILVSRLLLKKCGYLDEKVEKEIEVALSTTIRKGLGNNRSLCHGDFGQLEILRFAAEVLGDSYLQEVVNNLSGELYNLFKTEGYQSGTSRGTESVGLMVGLSGFGYGLLSAAYPSAVPSILTLDGEIQKYREPHEANH
Protein #2 69 residues (35% resolved)
MTNLLKEWKMPLERTHNNSNPAGDIFQELEDQDILAGVNGACAWYNISCRLGNKGAYCTLTVECMPSCN
MTNLLKEWKMPLERTHNNSNPAGDIFQELEDQDILAGVNGACAWYNISCRLGNKGAYCTLTVECMPSCN