2026-10-24_00000232|hard|9SX0

Structure of eMM762v1-sgRNA/DNA ternary complex; Electron microscopy 2.28Å
Protein #1 762 residues (87% resolved)

MEKELVLGIDYGGKYTGLAVVNQKNNQVLYARTVKMRDDVTDILAGRRRQRSLRRTLQTKKKRLRELKNYLESIGGIYEESSGTFTIEPFRTVYSLAHKRGYDYADLPEEKTSEEIEAMDAKERKQWEKEKKELEETQRNSRHRDEVLRDVRNVMTEGNLSEEQIIKVESIFNKQYRHKRFNNRILTKCKVCGKNTPLRRNVRELLLENIVRYLPLQNKERELLKLTILKGHQQDINEIFKHFRKVYKITLNQKDWPGKNLIDIARNQLRGRLLFCKVHFPENEKYVSIEKKTFRLAPSLKTKIENVLSVIKDDILPNFTLNNVVMESNNFDIAAKTKGKKRLLKEEYSKGHRESGETRKEALLRETDSRCIYCGKGIDLSNAHEAHIFPRKAGGINIFGNLVACCSVCNEEKRGRTPLESGILPKPEIVSRITNDLKKKILEDAQYINTLDFNKYMSHASIGWRHMRDRLRELTGNKELLIKRQSGIYTAYFRKWWGFIKERGNHGHHALDAVILASKKSYAEDGKVDMTIKPCGEDGKEFDIERHLSEMKEFRRDKGGKSAPLHDRNPLSFKNDIITRRFRVTEIECGKEAVIISEEYRKKLTEAFKRFGIAKGKYLTDEQAKDAGFYLRKNGEGVMSLKCEVKGTGYNQMIRIKNNIFKTNVHNVGVAVFLDEKGKKRACELKNPRLSKHFVKPAEQVKGKVIFILKRGNMVTVEGEEMIYRVKKLGTSPVIEAIVGSDGKTRRVSATKLLKINHTKKV

MEKELVLGIDYGGKYTGLAVVNQKNNQVLYARTVKMRDDVTDILAGRRRQRSLRRTLQTKKKRLRELKNYLESIGGIYEESSGTFTIEPFRTVYSLAHKRGYDYADLPEEKTSEEIEAMDAKERKQWEKEKKELEETQRNSRHRDEVLRDVRNVMTEGNLSEEQIIKVESIFNKQYRHKRFNNRILTKCKVCGKNTPLRRNVRELLLENIVRYLPLQNKERELLKLTILKGHQQDINEIFKHFRKVYKITLNQKDWPGKNLIDIARNQLRGRLLFCKVHFPENEKYVSIEKKTFRLAPSLKTKIENVLSVIKDDILPNFTLNNVVMESNNFDIAAKTKGKKRLLKEEYSKGHRESGETRKEALLRETDSRCIYCGKGIDLSNAHEAHIFPRKAGGINIFGNLVACCSVCNEEKRGRTPLESGILPKPEIVSRITNDLKKKILEDAQYINTLDFNKYMSHASIGWRHMRDRLRELTGNKELLIKRQSGIYTAYFRKWWGFIKERGNHGHHALDAVILASKKSYAEDGKVDMTIKPCGEDGKEFDIERHLSEMKEFRRDKGGKSAPLHDRNPLSFKNDIITRRFRVTEIECGKEAVIISEEYRKKLTEAFKRFGIAKGKYLTDEQAKDAGFYLRKNGEGVMSLKCEVKGTGYNQMIRIKNNIFKTNVHNVGVAVFLDEKGKKRACELKNPRLSKHFVKPAEQVKGKVIFILKRGNMVTVEGEEMIYRVKKLGTSPVIEAIVGSDGKTRRVSATKLLKINHTKKV
RNA #2 141 bases

GAGAACGGCGAAAACUAACUGUUACAGUUAAGGCUGCCUUAAUUGUAAAACGCCUAUACGGUGAGGGUAUGUACGUUUGGGUUUGUCCAGCCUAAACCUCUACGCCAGAAAUGGCACCUUCAUUGUGGGUUAGGACAAUUU

GAGAACGGCGAAAACUAACUGUUACAGUUAAGGCUGCCUUAAUUGUAAAACGCCUAUACGGUGAGGGUAUGUACGUUUGGGUUUGUCCAGCCUAAACCUCUACGCCAGAAAUGGCACCUUCAUUGUGGGUUAGGACAAUUU
DNA #3 35 bases

(DG)(DG)(DT)(DA)(DA)(DG)(DG)(DA)(DG)(DA)(DA)(DC)(DG)(DG)(DC)(DG)(DA)(DA)(DA)(DA)(DC)(DT)(DA)(DA)(DC)(DT)(DT)(DG)(DG)(DA)(DA)(DT)(DT)(DT)(DG)

GGTAAGGAGAACGGCGAAAACTAACTTGGAATTTG
DNA #4 29 bases

(DC)(DA)(DA)(DA)(DT)(DT)(DC)(DC)(DA)(DA)(DG)(DT)(DT)(DA)(DG)(DT)(DT)(DT)(DG)(DC)(DC)(DG)(DT)(DA)(DA)(DA)(DA)(DA)(DT)

CAAATTCCAAGTTAGTTTGCCGTAAAAAT
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Reference