2026-10-24_00000074|medium|21VR

Cryo-EM structure of E.coli BrxBCZ complex bound to ATPrS and Mg2+; Electron microscopy 3.03Å

Protein #1 743 residues (75% resolved)

Raw Sequence ...

MNIEQIFKKPLKRNINGVVKAEQTDDASAYIELDEYVITRELENHLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTERNAYSFFEEKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNERIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALSQATQQSLEASRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNTQMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQLSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKGDILRNQLAFDTTTTTALRPFTSEEEFVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSLVPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNLVTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDVDFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTPKDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPEQATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVIENT

Canonical Sequence ...

Protein #2 191 residues (100% resolved)

Raw Sequence ...

MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCELQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQLAGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMFYPGTYSGYNLSPLTDTGSQNYYRAFRLVPDT

Canonical Sequence ...

MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCELQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQLAGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMFYPGTYSGYNLSPLTDTGSQNYYRAFRLVPDT

Protein #3 84 residues (100% resolved)

Raw Sequence ...

KFAEHRIVFWHDPDKRFIEELEQLKLENVTLINMTHASQLAVKKRIEMDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHAD

Canonical Sequence ...

KFAEHRIVFWHDPDKRFIEELEQLKLENVTLINMTHASQLAVKKRIEMDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHAD

Non polymer #4 AGS

InChI=1S/C10H16N5O12P3S/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(25-10)1-24-28(18,19)26-29(20,21)27-30(22,23)31/h2-4,6-7,10,16-17H,1H2,(H,18,19)(H,20,21)(H2,11,12,13)(H2,22,23,31)/t4-,6-,7-,10-/m1/s1

InChI=1S/C10H16N5O12P3S/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(25-10)1-24-28(18,19)26-29(20,21)27-30(22,23)31/h2-4,6-7,10,16-17H,1H2,(H,18,19)(H,20,21)(H2,11,12,13)(H2,22,23,31)/t4-,6-,7-,10-/m1/s1

SMILES: c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=S)(O)O)O)O)N

c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=S)(O)O)O)O)N

Non polymer #5 MG

InChI=1S/Mg/q+2

SMILES: [Mg+2]

[Mg+2]

Biounit #1 hetero-2-1-1-mer

Reference