2026-10-10_00000217|hard|9ZFS

CryoEM structure of respiratory complex III intermediate from Acanthamoeba castellanii; Electron microscopy 3.65Å
Protein #1 502 residues (89% resolved)

MKPQHAARLSLAKGVTRASSPSASVLGSKSYSSWTATSSLKQAHSSVPQTWEATPQTLDNVQTTTLDSGLKVTTVSSELPLAAVGLHVKAGARFETGESRGAAHFLRHLAFSRTSSRSPLTVTREMEVATAAFDVSASRENISYSGQLMPDYLEDYVWMLRDLMHPLAWEYIVRDAAPQVAAEVHEAETNPATALVEAIHREAYRDEGLGNSIYCPNYRVGAVTREAIIRYHHERYQASNVALVGYGIKHEQLVAQANKYFPADAFAEDKAPWTTLEAADRKPGAVYTAASSYTGGELRLPGPGNSRVALAFEGASLADPDVFAVRTLSSLLGGAARFTRDGPGVGLRSRLARNVLAKGDYVLASSALNASYSDSGLFGVFVEALPGHGADAARLLSAELNSLAGSFSVDDAELTRAKNQAKASFFREVESRTGLVDYLARHTLAGTAPLAPAQYAARFDAVTRDDLARVARRVFSSPLTLVSTGDIHGVPTKEELRPKLKA

MKPQHAARLSLAKGVTRASSPSASVLGSKSYSSWTATSSLKQAHSSVPQTWEATPQTLDNVQTTTLDSGLKVTTVSSELPLAAVGLHVKAGARFETGESRGAAHFLRHLAFSRTSSRSPLTVTREMEVATAAFDVSASRENISYSGQLMPDYLEDYVWMLRDLMHPLAWEYIVRDAAPQVAAEVHEAETNPATALVEAIHREAYRDEGLGNSIYCPNYRVGAVTREAIIRYHHERYQASNVALVGYGIKHEQLVAQANKYFPADAFAEDKAPWTTLEAADRKPGAVYTAASSYTGGELRLPGPGNSRVALAFEGASLADPDVFAVRTLSSLLGGAARFTRDGPGVGLRSRLARNVLAKGDYVLASSALNASYSDSGLFGVFVEALPGHGADAARLLSAELNSLAGSFSVDDAELTRAKNQAKASFFREVESRTGLVDYLARHTLAGTAPLAPAQYAARFDAVTRDDLARVARRVFSSPLTLVSTGDIHGVPTKEELRPKLKA
Protein #2 67 residues (87% resolved)

MGVRWTTYTLSPFRQKMFPGFWTGLIPRQIKRWKYNFPRVAPPILAGIAIYTWGNWKHDQIARSHWY

MGVRWTTYTLSPFRQKMFPGFWTGLIPRQIKRWKYNFPRVAPPILAGIAIYTWGNWKHDQIARSHWY
Protein #3 491 residues (92% resolved)

MSSIRSRAPSMLLPTKLGTSGRLGAAARRLSSGQPQAARDVEKRLRLPEYLLNVPPTQVTALPNKFRVASEHKHGETATVGVWIDAGSVWETAENNGVAHFLEHLAFKGTKNRTKEQIEVEIENMGGQLNAYTSREQTVYHAHVFKKDVPKAVEIISDIIQNSNLKEDDVERERGVILREMEEVESQTEEVIFDHLHSIAFQNTSLGYTILGPEKNIKKIKREDLVSYVGKHYTAPRMVLSAAGAVDHDELVKLAEKHFSGLSSETNVDYSNREKLFDFTGSMVQVRDTSIPLVHTTVAAKSVGWSDPDYFTFLVLQQLVGSWDRSLGGAKNLSSNLAETFATEELAHSLMSFNTCYHETGLFGAYFVGEMERTSDAIFEVLREWVRIGSGVSEVEVERAKNKLKSTYLMQLDGTQAVAEDIGRQLLTLGRRMPAAEAFMRIDAIDAKKVREVAYTYLNDVDVAVAAVGSVDSGLFPDYNVLRGWTYWNRL

MSSIRSRAPSMLLPTKLGTSGRLGAAARRLSSGQPQAARDVEKRLRLPEYLLNVPPTQVTALPNKFRVASEHKHGETATVGVWIDAGSVWETAENNGVAHFLEHLAFKGTKNRTKEQIEVEIENMGGQLNAYTSREQTVYHAHVFKKDVPKAVEIISDIIQNSNLKEDDVERERGVILREMEEVESQTEEVIFDHLHSIAFQNTSLGYTILGPEKNIKKIKREDLVSYVGKHYTAPRMVLSAAGAVDHDELVKLAEKHFSGLSSETNVDYSNREKLFDFTGSMVQVRDTSIPLVHTTVAAKSVGWSDPDYFTFLVLQQLVGSWDRSLGGAKNLSSNLAETFATEELAHSLMSFNTCYHETGLFGAYFVGEMERTSDAIFEVLREWVRIGSGVSEVEVERAKNKLKSTYLMQLDGTQAVAEDIGRQLLTLGRRMPAAEAFMRIDAIDAKKVREVAYTYLNDVDVAVAAVGSVDSGLFPDYNVLRGWTYWNRL
Protein #4 122 residues (85% resolved)

MAARKGSSGFLSRISSSFKPVSGYQAYGLRLEDFYNAENPEIQEVLRRLPNKTKEERDLRIRRGHELHLKGTTLPESSWTTPEEDGQTYMEPYMSEVVQEIEERKDFRADFLLPVEKRHKRK

MAARKGSSGFLSRISSSFKPVSGYQAYGLRLEDFYNAENPEIQEVLRRLPNKTKEERDLRIRRGHELHLKGTTLPESSWTTPEEDGQTYMEPYMSEVVQEIEERKDFRADFLLPVEKRHKRK
Protein #5 82 residues (41% resolved)

MNALKSSGASNWVYQNLFKRNSSYFALILVGAVVVESGVSRTVDFWWNSVNKGRTWNDFKRTQLPKIQAKKAAAAEEEESDE

MNALKSSGASNWVYQNLFKRNSSYFALILVGAVVVESGVSRTVDFWWNSVNKGRTWNDFKRTQLPKIQAKKAAAAEEEESDE
Protein #6 70 residues (67% resolved)

MSENWAGCAIKKSLEEKAHPKCLKKWAEYDACGQRIKGDTTGEAQCSGQYFEYWHCVDALVANDLFKRLK

MSENWAGCAIKKSLEEKAHPKCLKKWAEYDACGQRIKGDTTGEAQCSGQYFEYWHCVDALVANDLFKRLK
Protein #7 305 residues (45% resolved)

MLRQRTLTSATGMAFRSQTRSFATSAGASAKRLHPNVLGLVIGAGAVGAAAGTLISRARCSDDVLVAPAYPWSHKSEWKSYDVASLRRGHQVYKEVCAACHSLNYIAWRNLIETLYTEEEVIAMAAETEVEDGPNDDGEMFTRPGKPTDYLPRPYPNEEAARSANAGAIPPDLTEMVKAREQGADYIFALLTGYMPAPAGLNVREGLYYNPYFPGGAIGMPPPLNDGMLEYEDGTEASVSQMAKDVSAFLCWTARPEQDERRVMGIKVLIYLGALFGCSWYIKRFKWSVLKNRKVQFPGKNWPTV

MLRQRTLTSATGMAFRSQTRSFATSAGASAKRLHPNVLGLVIGAGAVGAAAGTLISRARCSDDVLVAPAYPWSHKSEWKSYDVASLRRGHQVYKEVCAACHSLNYIAWRNLIETLYTEEEVIAMAAETEVEDGPNDDGEMFTRPGKPTDYLPRPYPNEEAARSANAGAIPPDLTEMVKAREQGADYIFALLTGYMPAPAGLNVREGLYYNPYFPGGAIGMPPPLNDGMLEYEDGTEASVSQMAKDVSAFLCWTARPEQDERRVMGIKVLIYLGALFGCSWYIKRFKWSVLKNRKVQFPGKNWPTV
Protein #8 385 residues (86% resolved)

MRFIKKYPLLNIVNSFLIDYPAPPNISYLWNFGALAAFCLGIQIVTGIFLAMFYVPSIESAFSSVQYIMRDVNYGWLIRYIHANGASFFFICVYIHVFRGLYYNSYITPRELLWNLGVTILIVMILTAFLGYVLPWGQMSFWAATVITSLFSAIPVVGEYIVIWLWGGFSVDNATLNRFFSLHYLLPFVIALLSLIHVAVLHESGSSNPLNIALSVANKVPFHPYFIFKDLLGIIFFLIVFCYAVFFKPDSIIDPINNVPANPLVTPTHIVPEWYFLPFYAILRSIPNKLGGVITLGLALIVLFLLPFITNNVFKGSFFEISKTILFWSFFSVCVLLGWIGFKPIEDPYLMLGQMLTVLYFFYFFSLAVIVPTLQAYTFKNVFAK

MRFIKKYPLLNIVNSFLIDYPAPPNISYLWNFGALAAFCLGIQIVTGIFLAMFYVPSIESAFSSVQYIMRDVNYGWLIRYIHANGASFFFICVYIHVFRGLYYNSYITPRELLWNLGVTILIVMILTAFLGYVLPWGQMSFWAATVITSLFSAIPVVGEYIVIWLWGGFSVDNATLNRFFSLHYLLPFVIALLSLIHVAVLHESGSSNPLNIALSVANKVPFHPYFIFKDLLGIIFFLIVFCYAVFFKPDSIIDPINNVPANPLVTPTHIVPEWYFLPFYAILRSIPNKLGGVITLGLALIVLFLLPFITNNVFKGSFFEISKTILFWSFFSVCVLLGWIGFKPIEDPYLMLGQMLTVLYFFYFFSLAVIVPTLQAYTFKNVFAK
Protein #9 95 residues (24% resolved)

MSIHKLDKTHATVNLEEAATRTGTIRSVPYHETIAYQRFYANVFTKATVFGKRLIPLTKNYAPTAFRWGALAGLAAAYVIEPSFLTKYFTKSESK

MSIHKLDKTHATVNLEEAATRTGTIRSVPYHETIAYQRFYANVFTKATVFGKRLIPLTKNYAPTAFRWGALAGLAAAYVIEPSFLTKYFTKSESK
Non polymer #10 HEM

InChI=1S/C34H34N4O4.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);/q;+2/p-2/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;

Cc1c2n3c(c1CCC(=O)O)C=C4C(=C(C5=[N]4[Fe]36[N]7=C(C=C8N6C(=C5)C(=C8C)C=C)C(=C(C7=C2)C)C=C)C)CCC(=O)O
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Reference