2026-10-03_00000140|medium|38XX

Crystal structure of a Formate acetyltransferase (PFL) from Megasphaera lornae with an acetylated active site cysteine; X-ray diffraction 1.86Å

Protein #1 772 residues (97% resolved)

Raw Sequence ...

MAHHHHHHMGTLEAQTQGPGSMVLENKGWEGFTGRLWREECNVRDFIQNNYQPYEGDERFLQAPTEATDILWGKVQEYQKAEREHGGVLKEETEIVSSITAYGPGYIDPQMKELEKVVGLQTDEPLKRAFMPYGGIKMAEEALEMYNYKLNPELHKVFTEYHKTHNEAVFDAYTKEMLQARHTHIITGLPDAYARGRIVGDYRRVALYGIDQLIAWKERDKEYNDDGIMTDNVIRLREEIAEQIKALKQMKELANIYGYDISKPATNAREAVQWLYFGYLAAIKTQNGAAMSVGRISTFLDIYMERDLAAGKLTEQEAQELIDHLTLKFRIVKFARIRSYNELFSGDPIWATLEMAGIGMDGRSMVTKTDYRFLHTLENMGPAPEPNLTVLYSSQLPHPFKKYAAHISDLTSSIQYENDDVMKPVWGDDYSIC(SCY)CVSATQTGKEMQFFGARANLAKALLYAINGGVDEKFGIQAGPELAPITSEYLDYDEVVHKYKLMLDWLAGLYVNVLNTIHYMHDKYYYEAAEMALLDTKLRRTFATGIAGFSHVIDSLSAIKYAKVKVIRNDKGLAEDFQVEGDFPKYGNDDDRADEIGVAVLREFMDDIRKRHTYRDSEATTSILTITSNVVYGKFTGNLPDGRRAWKPFAPGANPSYGAEEKGLMASLNSVAKLPYTWALDGISNTQSIIPEALGHNEEERAENLVHVLDGYFDQGAHHLNVNIFGKEKLLDAMEHPEKPEYANFTIRVSGYAVKFISLTKEQQLDVIARTFHEKR

Canonical Sequence ...

MAHHHHHHMGTLEAQTQGPGSMVLENKGWEGFTGRLWREECNVRDFIQNNYQPYEGDERFLQAPTEATDILWGKVQEYQKAEREHGGVLKEETEIVSSITAYGPGYIDPQMKELEKVVGLQTDEPLKRAFMPYGGIKMAEEALEMYNYKLNPELHKVFTEYHKTHNEAVFDAYTKEMLQARHTHIITGLPDAYARGRIVGDYRRVALYGIDQLIAWKERDKEYNDDGIMTDNVIRLREEIAEQIKALKQMKELANIYGYDISKPATNAREAVQWLYFGYLAAIKTQNGAAMSVGRISTFLDIYMERDLAAGKLTEQEAQELIDHLTLKFRIVKFARIRSYNELFSGDPIWATLEMAGIGMDGRSMVTKTDYRFLHTLENMGPAPEPNLTVLYSSQLPHPFKKYAAHISDLTSSIQYENDDVMKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKALLYAINGGVDEKFGIQAGPELAPITSEYLDYDEVVHKYKLMLDWLAGLYVNVLNTIHYMHDKYYYEAAEMALLDTKLRRTFATGIAGFSHVIDSLSAIKYAKVKVIRNDKGLAEDFQVEGDFPKYGNDDDRADEIGVAVLREFMDDIRKRHTYRDSEATTSILTITSNVVYGKFTGNLPDGRRAWKPFAPGANPSYGAEEKGLMASLNSVAKLPYTWALDGISNTQSIIPEALGHNEEERAENLVHVLDGYFDQGAHHLNVNIFGKEKLLDAMEHPEKPEYANFTIRVSGYAVKFISLTKEQQLDVIARTFHEKR

Non polymer #2 1PE

InChI=1S/C10H22O6/c11-1-3-13-5-7-15-9-10-16-8-6-14-4-2-12/h11-12H,1-10H2

SMILES: C(COCCOCCOCCOCCO)O

C(COCCOCCOCCOCCO)O

Non polymer #3 CL

InChI=1S/ClH/h1H/p-1

SMILES: [Cl-]

[Cl-]

Non polymer #4 MPD

InChI=1S/C6H14O2/c1-5(7)4-6(2,3)8/h5,7-8H,4H2,1-3H3/t5-/m0/s1

SMILES: C[C@@H](CC(C)(C)O)O

C[C@@H](CC(C)(C)O)O

Non polymer #5 NO3

InChI=1S/NO3/c2-1(3)4/q-1

SMILES: [N+](=O)([O-])[O-]

[N+](=O)([O-])[O-]

Non polymer #6 PG4

InChI=1S/C8H18O5/c9-1-3-11-5-7-13-8-6-12-4-2-10/h9-10H,1-8H2

SMILES: C(COCCOCCOCCO)O

C(COCCOCCOCCO)O

Non polymer #7 PO4

InChI=1S/H3O4P/c1-5(2,3)4/h(H3,1,2,3,4)/p-3

SMILES: [O-]P(=O)([O-])[O-]

[O-]P(=O)([O-])[O-]

Biounit #1 homo-tetramer

Reference