2026-08-29_00000052|hard|23VU

Cryo-EM structure of the UnCas12m4a-crRNA-tgDNA ternary complex in the TS-cleaving state; Electron microscopy 2.69Å
Protein #1 614 residues (97% resolved)

MGHHHHHHGGMAVEERTKKGVSVRVYKYGLVPKGCLPDEAKDELLRANNLWNKLVEISRKNQSDFDEVRKKAHPPYGEEMVRLETINEKIDDAYDRKRDARKEAGTRDETHPLIIKANAVIEILKEERSEIYDTLKLLRTEADKSVDKKALNESFKSNIKLARRETSLNSDTKEEIIRNDFRTARDRTFKTGGRLRFHTFDGTGYWHFRFRRNDEKGKKVDDYTIDDLFAGEKPSPKKPLPNRFKFLSRDDTRRKPRLRLRTTLAGGRTNASKVFQEFDVIYHRPVPEGAIIPNAKILRTRTGDKFRYDLVLTVYLPEPKHKDIPLDDAIGIDLGFREDPDDKYRKQVGAIISLDPSDEVEEIFAPPKMVKAFGHIDELKSVLDESAADLGMKIKPLMKDIRLPEDNEKYKQEYKLWNSIVNARANVTLSFEKAYKLALWCKKKDAGIPEDITGPVVYWWKGYSRRYRELHNLRKKQLLNRKDFYRQIASRLVLRGLLIGVENFNLSKIARSKDEDNVLNNKARANRFLLSPSEFRAAIKNAADREGIPCLEVNPANTSKICFDCGTLNKNLGSEKNWVCPACGCVHDRDTNAARNIAKRALEKYLEDRKSAGK

MGHHHHHHGGMAVEERTKKGVSVRVYKYGLVPKGCLPDEAKDELLRANNLWNKLVEISRKNQSDFDEVRKKAHPPYGEEMVRLETINEKIDDAYDRKRDARKEAGTRDETHPLIIKANAVIEILKEERSEIYDTLKLLRTEADKSVDKKALNESFKSNIKLARRETSLNSDTKEEIIRNDFRTARDRTFKTGGRLRFHTFDGTGYWHFRFRRNDEKGKKVDDYTIDDLFAGEKPSPKKPLPNRFKFLSRDDTRRKPRLRLRTTLAGGRTNASKVFQEFDVIYHRPVPEGAIIPNAKILRTRTGDKFRYDLVLTVYLPEPKHKDIPLDDAIGIDLGFREDPDDKYRKQVGAIISLDPSDEVEEIFAPPKMVKAFGHIDELKSVLDESAADLGMKIKPLMKDIRLPEDNEKYKQEYKLWNSIVNARANVTLSFEKAYKLALWCKKKDAGIPEDITGPVVYWWKGYSRRYRELHNLRKKQLLNRKDFYRQIASRLVLRGLLIGVENFNLSKIARSKDEDNVLNNKARANRFLLSPSEFRAAIKNAADREGIPCLEVNPANTSKICFDCGTLNKNLGSEKNWVCPACGCVHDRDTNAARNIAKRALEKYLEDRKSAGK
RNA #2 56 bases

GGAUAACGACCCUGCGAAGUGGGGAGUAACUUCGACGUAAAUUAGCUGCGCUUCAC

GGAUAACGACCCUGCGAAGUGGGGAGUAACUUCGACGUAAAUUAGCUGCGCUUCAC
DNA #3 39 bases

(DT)(DG)(DA)(DA)(PST)(SC)(GS)(PST)(PST)(GS)(PST)(GS)(DA)(DA)(DG)(DC)(DG)(DC)(DA)(DG)(DC)(DT)(DA)(DA)(DT)(DT)(DT)(DA)(DC)(DC)(DG)(DG)(DT)(DT)(DA)(DG)(DA)(DA)(DA)

TGAATCGTTGTGAAGCGCAGCTAATTTACCGGTTAGAAA
DNA #4 39 bases

(DT)(DT)(DT)(DC)(DT)(DA)(DA)(DC)(DC)(DG)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DG)(DA)(DT)(DT)(DC)(DA)

TTTCTAACCGTTTTTTTTTTTTTTTTTTTTAACGATTCA
Non polymer #5 MG

InChI=1S/Mg/q+2

[Mg+2]
Non polymer #6 ZN

InChI=1S/Zn/q+2

[Zn+2]
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Reference