2026-07-11_00000013|medium|10ZN

CryoEM structure of Heterologous nitrogenase complex (2:1 A. vinelandii-FeP:G. diazotrophicus-MoFeP) inhibited by BeFx (C2 symmetry); Electron microscopy 2.92Å
Protein #1 499 residues (97% resolved)

MSLDEDKTNDSAFHARLIAEVLEAYPDKARKRRQKHLNVAGQAEAEAQDAGEEGVMLSECDVKSNVKSVPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYIGNTGVDSFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIDELFPLAKGISVQSECPIGLIGDDIEAVSRKKKKEIGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDGEDKHAAFETTPYDVNVIGDYNIGGDAWSSRILLEEMGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYNIPWTEYNFFGPSQIAASLRKIAALFDEKIQEGAERVIAKYQPLVDAVIEKFRPRLAGKKVMLYVGGLRPRHVVNAYNDLGMEIVGTGYEFGHNDDYQRTGHYVREGTLIYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVWSMFKAPWKNAA

MSLDEDKTNDSAFHARLIAEVLEAYPDKARKRRQKHLNVAGQAEAEAQDAGEEGVMLSECDVKSNVKSVPGVMTIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYYIGNTGVDSFVTMQFTSDFQEKDIVFGGDKKLEKIIDEIDELFPLAKGISVQSECPIGLIGDDIEAVSRKKKKEIGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDGEDKHAAFETTPYDVNVIGDYNIGGDAWSSRILLEEMGLRVVGNWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYNIPWTEYNFFGPSQIAASLRKIAALFDEKIQEGAERVIAKYQPLVDAVIEKFRPRLAGKKVMLYVGGLRPRHVVNAYNDLGMEIVGTGYEFGHNDDYQRTGHYVREGTLIYDDVTGYELEKFIEGIRPDLVGSGIKEKYPVQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDLAINNPVWSMFKAPWKNAA
Protein #2 511 residues (100% resolved)

MPQNVDKILDHAPLFREPEYQEMLAGKAKLENMPPADKVVEIADWTKSWEYREKNFARESLSVNPAKACQPLGAVFVASGFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLNNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDLHAFIQTAKGKGSVPEEFDVPFAHTPAFVGSHVTGYDNMLKGILEHFWKGRTPVPNRSVNIIPGFDGFAVGNNRELKRILGMMGVQYTILSDVSDQFDTPSDGEYRMYDGGTKIEAARDAVNADYTISLQEYCTPKTLEYCQSFGQKTASFHYPLGIGATDDLLQKLSEISGKPVPQELEMERGRLVDALADSQAYLHGKTYAIYGDPDFVYGMARFILETGGEPKHCLATNGSKAWEAQMQELFDSSPFGVGCKAWGGKDLWHMRSLLATEKVDLLIGNSYGKYLERDTDTPLIRLMFPIFDRHHHHRFPVWGYQGALRVLVTLLDKIFDKLDDDTIQAGVTDYSFDLTR

MPQNVDKILDHAPLFREPEYQEMLAGKAKLENMPPADKVVEIADWTKSWEYREKNFARESLSVNPAKACQPLGAVFVASGFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLNNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDLHAFIQTAKGKGSVPEEFDVPFAHTPAFVGSHVTGYDNMLKGILEHFWKGRTPVPNRSVNIIPGFDGFAVGNNRELKRILGMMGVQYTILSDVSDQFDTPSDGEYRMYDGGTKIEAARDAVNADYTISLQEYCTPKTLEYCQSFGQKTASFHYPLGIGATDDLLQKLSEISGKPVPQELEMERGRLVDALADSQAYLHGKTYAIYGDPDFVYGMARFILETGGEPKHCLATNGSKAWEAQMQELFDSSPFGVGCKAWGGKDLWHMRSLLATEKVDLLIGNSYGKYLERDTDTPLIRLMFPIFDRHHHHRFPVWGYQGALRVLVTLLDKIFDKLDDDTIQAGVTDYSFDLTR
Protein #3 290 residues (93% resolved)

MAMRQCAIYGKGGIGKSTTTQNLVAALAEMGKKVMIVGCDPKADSTRLILHSKAQNTIMEMAAEAGTVEDLELEDVLKAGYGGVKCVESGGPEPGVGCAGRGVITAINFLEEEGAYEDDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNISKGIVKYANSGSVRLGGLICNSRNTDREDELIIALANKLGTQMIHFVPRDNVVQRAEIRRMTVIEYDPKAKQADEYRALARKVVDNKLLVIPNPITMDELEELLMEFGIMEVEDESIVGKTAEEV

MAMRQCAIYGKGGIGKSTTTQNLVAALAEMGKKVMIVGCDPKADSTRLILHSKAQNTIMEMAAEAGTVEDLELEDVLKAGYGGVKCVESGGPEPGVGCAGRGVITAINFLEEEGAYEDDLDFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNISKGIVKYANSGSVRLGGLICNSRNTDREDELIIALANKLGTQMIHFVPRDNVVQRAEIRRMTVIEYDPKAKQADEYRALARKVVDNKLLVIPNPITMDELEELLMEFGIMEVEDESIVGKTAEEV
Non polymer #4 0BE

InChI=1S/Be/q+2

[Be+2]
Non polymer #5 ADP

InChI=1S/C10H15N5O10P2/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(24-10)1-23-27(21,22)25-26(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1

c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@](=O)(O)OP(=O)(O)O)O)O)N
Non polymer #6 CLF

InChI=1S/8Fe.7S

[S]12[Fe]3[S]4[Fe]1S567([Fe]2[S]3[Fe]45)[Fe]8[S]9[Fe]6[S]1[Fe]9[S]8[Fe]71
Non polymer #7 FE

InChI=1S/Fe/q+3

[Fe+3]
Non polymer #8 HCA

InChI=1S/C7H10O7/c8-4(9)1-2-7(14,6(12)13)3-5(10)11/h14H,1-3H2,(H,8,9)(H,10,11)(H,12,13)/t7-/m1/s1

C(C[C@@](CC(=O)O)(C(=O)O)O)C(=O)O
Non polymer #9 ICS

InChI=1S/C.7Fe.Mo.9S

C12345[Fe]67S[Fe]18[S]9[Fe]21S[Fe]32[S]6[Fe]3[S]7[Fe]4([S]23)S[Fe]52[S]8[Mo]9[S]12
Non polymer #10 MG

InChI=1S/Mg/q+2

[Mg+2]
Non polymer #11 SF4

InChI=1S/4Fe.4S

[S]12[Fe]3[S]4[Fe]1[S]5[Fe]2[S]3[Fe]45
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Reference