2026-06-27_00000048|hard|21EA

Cryo-EM structure of AtCas9-sgRNA-underwound DNA (TATA PAM) ternary complex; Electron microscopy 2.65Å
Protein #1 1154 residues (73% resolved)

MAYRLGLAIGITSVGWAVVALEKDESGLKPVRIQDLGVRIFDKAEDSKTGASLALPRREARSARRRTRRRRHRLWRVKRLLEQHGILSMEQIEALYAQRTSSPDVYALRVAGLDRCLIAEEIARVLIHIAHRRGFQSNRKSEIKDSDAGKLLKAVQENENLMQSKGYRTVAEMLVSEATKTDAEGKLVHGKKHGYVSNVRNKAGEYRHTVSRQAIVDEVRKIFAAQRALGNDVMSEELEDSYLKILCSQRNFDDGPGGDSPYGHGSVSPDGVRQSIYERMVGSCTFETGEKRAPRSSYSFERFQLLTKVVNLRIYRQQEDGGRYPCELTQTERARVIDCAYEQTKITYGKLRKLLDMKDTESFAGLTYGLNRSRNKTEDTVFVEMKFYHEVRKALQRAGVFIQDLSIETLDQIGWILSVWKSDDNRRKKLSTLGLSDNVIEELLPLNGSKFGHLSLKAIRKILPFLEDGYSYDVACELAGYQFQGKTEYVKQRLLPPLGEGEVTNPVVRRALSQAIKVVNAVIRKHGSPESIHIELARELSKNLDERRKIEKAQKENQKNNEQIKDEIREILGSAHVTGRDIVKYKLFKQQQEFCMYSGEKLDVTRLFEPGYAEVDAIIPYGISFDDSYDNKVLVKTEQARQKGNRTPLEYLRDKPEQKAKFIALVESIPLSQKKKNHLLMDKRAIDLEQEGFRERNLSDTRYITRALMNHIQAWLLFDETASTRSKRVVCVNGAVTAYMRARWGLTKDRNAGDKHHAADAVVVACIGDSLIQRVTKYDKFKRNALADRNRYVQQVSKSEGITQYVDKETGEVFTWESFDERKFLPNEPLEPWPFFRDELLARLSDDPSKNIRAIGLLTYSETEQIDPIFVSRMPTRKVTGAAHKETIRSPRIVKVDDNKGTEIQVVVSKVALTELKLTKDGEIKDYFRPEDDPRLYNTLRERLVQFGGDAKAAFKEPVYKISKDGSVRTPVRKVKIQEKLTLGVPVHGGRGIAENGGMVRIDVFAKDGKYYFVPIYVADVLKRELPNRLATAHKPYSEWRVVDDSYQFKFSLYPNDAVMIKPSREVDITYKDRKEPVGLRLMYFAGADISGATLTLRTHDNSGELKGLGIQGLEVFEKYVVGPLGDTHPVYKERRMPFRVERKMNLEHHHHHH

MAYRLGLAIGITSVGWAVVALEKDESGLKPVRIQDLGVRIFDKAEDSKTGASLALPRREARSARRRTRRRRHRLWRVKRLLEQHGILSMEQIEALYAQRTSSPDVYALRVAGLDRCLIAEEIARVLIHIAHRRGFQSNRKSEIKDSDAGKLLKAVQENENLMQSKGYRTVAEMLVSEATKTDAEGKLVHGKKHGYVSNVRNKAGEYRHTVSRQAIVDEVRKIFAAQRALGNDVMSEELEDSYLKILCSQRNFDDGPGGDSPYGHGSVSPDGVRQSIYERMVGSCTFETGEKRAPRSSYSFERFQLLTKVVNLRIYRQQEDGGRYPCELTQTERARVIDCAYEQTKITYGKLRKLLDMKDTESFAGLTYGLNRSRNKTEDTVFVEMKFYHEVRKALQRAGVFIQDLSIETLDQIGWILSVWKSDDNRRKKLSTLGLSDNVIEELLPLNGSKFGHLSLKAIRKILPFLEDGYSYDVACELAGYQFQGKTEYVKQRLLPPLGEGEVTNPVVRRALSQAIKVVNAVIRKHGSPESIHIELARELSKNLDERRKIEKAQKENQKNNEQIKDEIREILGSAHVTGRDIVKYKLFKQQQEFCMYSGEKLDVTRLFEPGYAEVDAIIPYGISFDDSYDNKVLVKTEQARQKGNRTPLEYLRDKPEQKAKFIALVESIPLSQKKKNHLLMDKRAIDLEQEGFRERNLSDTRYITRALMNHIQAWLLFDETASTRSKRVVCVNGAVTAYMRARWGLTKDRNAGDKHHAADAVVVACIGDSLIQRVTKYDKFKRNALADRNRYVQQVSKSEGITQYVDKETGEVFTWESFDERKFLPNEPLEPWPFFRDELLARLSDDPSKNIRAIGLLTYSETEQIDPIFVSRMPTRKVTGAAHKETIRSPRIVKVDDNKGTEIQVVVSKVALTELKLTKDGEIKDYFRPEDDPRLYNTLRERLVQFGGDAKAAFKEPVYKISKDGSVRTPVRKVKIQEKLTLGVPVHGGRGIAENGGMVRIDVFAKDGKYYFVPIYVADVLKRELPNRLATAHKPYSEWRVVDDSYQFKFSLYPNDAVMIKPSREVDITYKDRKEPVGLRLMYFAGADISGATLTLRTHDNSGELKGLGIQGLEVFEKYVVGPLGDTHPVYKERRMPFRVERKMNLEHHHHHH
RNA #2 117 bases

CAUACACUCUACCCCAACGAAUAAGUCAUAGUUCCCUCAGAAACGAGGUUGCUAUGAUAAGGCCGAGCAACAGGCUCGUGCCGCAAAGCACUGACCCCAAUGGGUCAUCUACUUUUU

CAUACACUCUACCCCAACGAAUAAGUCAUAGUUCCCUCAGAAACGAGGUUGCUAUGAUAAGGCCGAGCAACAGGCUCGUGCCGCAAAGCACUGACCCCAAUGGGUCAUCUACUUUUU
DNA #3 35 bases

(DC)(DT)(DG)(DT)(DA)(DT)(DA)(DG)(DT)(DA)(DC)(DT)(DT)(DA)(DT)(DT)(DC)(DG)(DT)(DT)(DG)(DG)(DG)(DG)(DT)(DA)(DG)(DA)(DG)(DT)(DG)(DT)(DA)(DT)(DG)

CTGTATAGTACTTATTCGTTGGGGTAGAGTGTATG
DNA #4 35 bases

(DC)(DA)(DT)(DA)(DC)(DA)(DC)(DT)(DC)(DT)(DA)(DC)(DC)(DC)(DC)(DA)(DA)(DC)(DG)(DA)(DA)(DT)(DA)(DA)(DG)(DT)(DA)(DC)(DT)(DA)(DT)(DA)(DC)(DA)(DG)

CATACACTCTACCCCAACGAATAAGTACTATACAG
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Reference