2026-02-21_00000057|hard|9O7O

CryoEM structure of M. mazei topoisomerase VI(A-E342Q)-minicircle DNA complex in asymmetric state; Electron microscopy 2.70Å
Protein #1 369 residues (98% resolved)

MEGEKGSKTRKGDALAREKLLEIAEKIYNQFEEEVVPSVSLPSRTKANLEYSDESDVWVYGDRESERSAKTVKGAFQLLKTTYATDFLINEHLARNRGSTLRELYYISEGWDYAKFKEQGESDRLIEDLEILTSLQREYFHMRPEEDGATMFGPIEITEQTKRGERNIHCQKDVGEGGYQIPFNVENIEFQKHDASMIIAIETGGMYARLMENGFDEAYNAILVHLKGQPARSTRRIIKRMNEELGIPVAVFTDGDPWSYRIYASVAYGAIKSAHLSEFMATPAAKFLGLQPSDIVEYELSTDKLTEQDVSALRSELSDPRFESDYWKEQIQLQLDIGKKAQQQAFAGKGLDFVTEVYLPNRLKEMGMI

MEGEKGSKTRKGDALAREKLLEIAEKIYNQFEEEVVPSVSLPSRTKANLEYSDESDVWVYGDRESERSAKTVKGAFQLLKTTYATDFLINEHLARNRGSTLRELYYISEGWDYAKFKEQGESDRLIEDLEILTSLQREYFHMRPEEDGATMFGPIEITEQTKRGERNIHCQKDVGEGGYQIPFNVENIEFQKHDASMIIAIETGGMYARLMENGFDEAYNAILVHLKGQPARSTRRIIKRMNEELGIPVAVFTDGDPWSYRIYASVAYGAIKSAHLSEFMATPAAKFLGLQPSDIVEYELSTDKLTEQDVSALRSELSDPRFESDYWKEQIQLQLDIGKKAQQQAFAGKGLDFVTEVYLPNRLKEMGMI
Protein #2 621 residues (98% resolved)

METPIAEELAKKQKSISVAEFFEKNRQILGFDSAPRSLITTVKEAVDNALDACEEAGILPDILVQVERTGPDYVTVIIEDNGPGIVREQIPKVFAKLLYGSRFHALKQSRGQQGIGISAAVLYAQMTAGRHTKILSKTSPTAPAHYYELMINTSTNEPDILVDEVRDWFRPHGTQIELEMRAAYVKGRRQSIYEYLKATAIVNPHARITLIDPDGNEEVFERATDKMPEPAEEILPHPEGIELGTLMKMLHYTERQKLAPFLRYSFCKIGLLTAEEICKAAGLDPEIDPHALGRHEARKLIEAFEKVKIMAPPTDCLSPIGEDLIYRGLEKETTVDFIATSTRKPAVYSGNPFVVEVGMAYGGNLPKEEKISIMRFANRVPLLYQQGGCVTTHAVEDIKWKQYGLNQPGGGIPVGPVILLIHVASINVPFTSESKDAIADIPVIKEEIDLAIKEVARKLKHYLSKQSNLKKRREKEIIITKVLPKLAAKVAHVLEKDVPDINPVVAKIMGNLLVHRVIKNNGDGTVDVAIKVKNFGTSAYSFRVHEMLPCKVSGAKPEPKVVTMGNDYDYVWDISASAGSSKVLSYKIESASEEELQKLPQLIVEGIEEELVTGAKAFKGV

METPIAEELAKKQKSISVAEFFEKNRQILGFDSAPRSLITTVKEAVDNALDACEEAGILPDILVQVERTGPDYVTVIIEDNGPGIVREQIPKVFAKLLYGSRFHALKQSRGQQGIGISAAVLYAQMTAGRHTKILSKTSPTAPAHYYELMINTSTNEPDILVDEVRDWFRPHGTQIELEMRAAYVKGRRQSIYEYLKATAIVNPHARITLIDPDGNEEVFERATDKMPEPAEEILPHPEGIELGTLMKMLHYTERQKLAPFLRYSFCKIGLLTAEEICKAAGLDPEIDPHALGRHEARKLIEAFEKVKIMAPPTDCLSPIGEDLIYRGLEKETTVDFIATSTRKPAVYSGNPFVVEVGMAYGGNLPKEEKISIMRFANRVPLLYQQGGCVTTHAVEDIKWKQYGLNQPGGGIPVGPVILLIHVASINVPFTSESKDAIADIPVIKEEIDLAIKEVARKLKHYLSKQSNLKKRREKEIIITKVLPKLAAKVAHVLEKDVPDINPVVAKIMGNLLVHRVIKNNGDGTVDVAIKVKNFGTSAYSFRVHEMLPCKVSGAKPEPKVVTMGNDYDYVWDISASAGSSKVLSYKIESASEEELQKLPQLIVEGIEEELVTGAKAFKGV
DNA #3 74 bases

(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DA)

TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
Non polymer #4 ANP

InChI=1S/C10H17N6O12P3/c11-8-5-9(13-2-12-8)16(3-14-5)10-7(18)6(17)4(27-10)1-26-31(24,25)28-30(22,23)15-29(19,20)21/h2-4,6-7,10,17-18H,1H2,(H,24,25)(H2,11,12,13)(H4,15,19,20,21,22,23)/t4-,6-,7-,10-/m1/s1

c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@](=O)(O)O[P@@](=O)(NP(=O)(O)O)O)O)O)N
Non polymer #5 CA

InChI=1S/Ca/q+2

[Ca+2]
Non polymer #6 K

InChI=1S/K/q+1

[K+]
Download coordinates
Reference