2025-12-20_00000156|hard|9N9M

Cryo-EM structure of the dCas12f-gRNA-DNA complex (partial R-Loop); Electron microscopy 3.38Å
Protein #1 365 residues (100% resolved)

MGKSTLKHTRKIQILIDLPTKDEKKEVMDMMYQWRDRCFRAANIIVTHLYVQEMIKDFFYLSEGIKYKLADEKKDEKGILQRSRMNTTYRVVSDRFKGEMPTNILSTLNHGLISSFNKNRVQYWKGERSLPNFKKDMAFPFGLQGISRLVYDEEKKAFCFRLYRVPFKTYLGKDFTDKRMLLERLVKGDVKLCASNIQLNGGKIFWLAVFEIEKEKHSLKPEVIAEASLSLEYPIVVKTGKNRLTIGTKEEFLYRRLAIQAARRRTQVGATYSRSGKGKKRKLKAVDKYHKTESNYVAHRIHVYSRKLIDFCIKHQAGTLILMNQEDKVGIAKEEEFVLRNWSYYELMTKIKYKAEKAGIELIIG

MGKSTLKHTRKIQILIDLPTKDEKKEVMDMMYQWRDRCFRAANIIVTHLYVQEMIKDFFYLSEGIKYKLADEKKDEKGILQRSRMNTTYRVVSDRFKGEMPTNILSTLNHGLISSFNKNRVQYWKGERSLPNFKKDMAFPFGLQGISRLVYDEEKKAFCFRLYRVPFKTYLGKDFTDKRMLLERLVKGDVKLCASNIQLNGGKIFWLAVFEIEKEKHSLKPEVIAEASLSLEYPIVVKTGKNRLTIGTKEEFLYRRLAIQAARRRTQVGATYSRSGKGKKRKLKAVDKYHKTESNYVAHRIHVYSRKLIDFCIKHQAGTLILMNQEDKVGIAKEEEFVLRNWSYYELMTKIKYKAEKAGIELIIG
DNA #2 60 bases

(DC)(DT)(DA)(DG)(DC)(DG)(DT)(DT)(DG)(DT)(DG)(DG)(DG)(DA)(DT)(DA)(DC)(DC)(DC)(DG)(DT)(DT)(DA)(DC)(DA)(DC)(DT)(DA)(DA)(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DG)(DT)(DT)(DC)(DA)(DC)(DG)(DA)(DT)(DA)(DT)(DC)(DC)(DT)(DT)(DT)(DA)(DG)(DT)(DC)(DC)(DC)(DA)(DC)

CTAGCGTTGTGGGATACCCGTTACACTAATTTTTCGGTTCACGATATCCTTTAGTCCCAC
DNA #3 60 bases

(DG)(DT)(DG)(DG)(DG)(DA)(DC)(DT)(DA)(DA)(DA)(DG)(DG)(DA)(DT)(DA)(DT)(DC)(DG)(DT)(DG)(DA)(DA)(DC)(DC)(DG)(DA)(DA)(DA)(DA)(DA)(DT)(DT)(DA)(DG)(DT)(DG)(DT)(DA)(DA)(DC)(DG)(DG)(DG)(DT)(DA)(DT)(DC)(DC)(DC)(DA)(DC)(DA)(DA)(DC)(DG)(DC)(DT)(DA)(DG)

GTGGGACTAAAGGATATCGTGAACCGAAAAATTAGTGTAACGGGTATCCCACAACGCTAG
RNA #4 93 bases

AUUUUGAGGGUGCUUGUACACCCAUAGGGUGAGGUUAAGAAUUACACUCACUAAGUGUGAACAACACAUACAACUUGUGGGAUAUACGCUAAC

AUUUUGAGGGUGCUUGUACACCCAUAGGGUGAGGUUAAGAAUUACACUCACUAAGUGUGAACAACACAUACAACUUGUGGGAUAUACGCUAAC
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Reference