2025-11-22_00000162|hard|9LQ8

Cryo-EM structure of CTR-bound type III-B CRISPR-Cas effector complex; Electron microscopy 2.85Å
Protein #1 469 residues (100% resolved)

MNQLTAILKQHTPMIHFQHNESGATLRASEVKPLLDKFILTKLGNGDIREGRLYAKKNNWLIDNEKNYALNYKLSISLQKKSRLEYLITSSTFPLPTERPSNFFTIQNSPYFAQEKCVGINTNSTIILKKSNSDPRKKEAEFKEKNWSQIDKKGLEWQDFTIKIFSLKGDLINKIQTYLPAFFICHNFGTRNNKGFGSFTVEYINNQKNICNVEDTLKENFAFVYKKKIALSRQSTLDFIYIYNQIFSTIKKDYQILKSGYNFRNEYIKSLLFCYFVSKYPNYRWEKRKMKQLIKARGYELKGDHSPISGIRENDNSWNDPNPNGYNYAYIRAILGLAEQYEFQLETPYQKAIVKIKSANNCISRYKSPLLFKIINNSIYLVGNEINTEILNKPFQYSYIEQTKNKNMRTGKSEITERTMHINEIEMNYKNRINYHYTPTSFSLIDFMQYAMSYKKNGKNILNYIPLKQ

MNQLTAILKQHTPMIHFQHNESGATLRASEVKPLLDKFILTKLGNGDIREGRLYAKKNNWLIDNEKNYALNYKLSISLQKKSRLEYLITSSTFPLPTERPSNFFTIQNSPYFAQEKCVGINTNSTIILKKSNSDPRKKEAEFKEKNWSQIDKKGLEWQDFTIKIFSLKGDLINKIQTYLPAFFICHNFGTRNNKGFGSFTVEYINNQKNICNVEDTLKENFAFVYKKKIALSRQSTLDFIYIYNQIFSTIKKDYQILKSGYNFRNEYIKSLLFCYFVSKYPNYRWEKRKMKQLIKARGYELKGDHSPISGIRENDNSWNDPNPNGYNYAYIRAILGLAEQYEFQLETPYQKAIVKIKSANNCISRYKSPLLFKIINNSIYLVGNEINTEILNKPFQYSYIEQTKNKNMRTGKSEITERTMHINEIEMNYKNRINYHYTPTSFSLIDFMQYAMSYKKNGKNILNYIPLKQ
Protein #2 423 residues (87% resolved)

MSHHHHHHHHDGKPIPNPLLGLDSTGSDQTENSGENLYFQGANAMNRHYLITLTPMDWFFFGGERTLDDGKSADYISHSNKFPQQSALLGMIRYQLLKQHNLLSQFPYTENKPTEKEIMKTLIGEQSFRMTERKAKSLGLGVIKQISPLMLIECKDDTSSRSIYFPLPLDDGYKVSFNETSNEDKVFYNGIECPIPNVYPASEEQDSGNQKRKFFDHKTYNNYLFWCTQGNNQIKKLLSDEIWISKMQIGITKHVEEGEDNDKSFYKQEFLQLKKSFIYAFYITLSGESELSSDIIQLGGQRSVFRMEVESIEENSDIQEKYQTAAQFLTQSDRLLILSPTYVDNLKELSALCNFMWSDSIVFRNIQTTNASNFYGKPIKSSSKYHFLKPGSVLYFKQGKRKEVEKLLMDYTYLRLSGYNIYI

MSHHHHHHHHDGKPIPNPLLGLDSTGSDQTENSGENLYFQGANAMNRHYLITLTPMDWFFFGGERTLDDGKSADYISHSNKFPQQSALLGMIRYQLLKQHNLLSQFPYTENKPTEKEIMKTLIGEQSFRMTERKAKSLGLGVIKQISPLMLIECKDDTSSRSIYFPLPLDDGYKVSFNETSNEDKVFYNGIECPIPNVYPASEEQDSGNQKRKFFDHKTYNNYLFWCTQGNNQIKKLLSDEIWISKMQIGITKHVEEGEDNDKSFYKQEFLQLKKSFIYAFYITLSGESELSSDIIQLGGQRSVFRMEVESIEENSDIQEKYQTAAQFLTQSDRLLILSPTYVDNLKELSALCNFMWSDSIVFRNIQTTNASNFYGKPIKSSSKYHFLKPGSVLYFKQGKRKEVEKLLMDYTYLRLSGYNIYI
Protein #3 279 residues (100% resolved)

MTTRMYVINTLSNMHVGSGEVNYGVIANLIQRDSVTNLPNINSSGLKGAIREYFKENEDLVRELFGSAPRDEKTLPGKVRFFEANLLSMPVRSDKVPFLMAISDEVLQELITKMKFFNCEEATQYISHLSTLLDNIKTQAQGTDFAYVFDPLLQGAIIEEVSIRATCPSHIPLQPSLKKLLGDRLVILSHKYFSILSDDNHLPVLSRNNLENGQSANLWYEQVLPRYSRLYFMLMDGNAQSEYLKKFRDTLCTPSTIIQIGANASIGYGYCQISELSPF

MTTRMYVINTLSNMHVGSGEVNYGVIANLIQRDSVTNLPNINSSGLKGAIREYFKENEDLVRELFGSAPRDEKTLPGKVRFFEANLLSMPVRSDKVPFLMAISDEVLQELITKMKFFNCEEATQYISHLSTLLDNIKTQAQGTDFAYVFDPLLQGAIIEEVSIRATCPSHIPLQPSLKKLLGDRLVILSHKYFSILSDDNHLPVLSRNNLENGQSANLWYEQVLPRYSRLYFMLMDGNAQSEYLKKFRDTLCTPSTIIQIGANASIGYGYCQISELSPF
Protein #4 136 residues (100% resolved)

MKISKKQIEYAIEALRANNIITNDNQYPKVFKGYISSFGAAVIQSGLIPAIIFFENEDNDANADRHKIIGVLKDIINAMRQQYTVTDATILVSSQIPANYSMAQYIIEHGNTDQLLKEITEAAVAMKLALRMYKSE

MKISKKQIEYAIEALRANNIITNDNQYPKVFKGYISSFGAAVIQSGLIPAIIFFENEDNDANADRHKIIGVLKDIINAMRQQYTVTDATILVSSQIPANYSMAQYIIEHGNTDQLLKEITEAAVAMKLALRMYKSE
Protein #5 314 residues (100% resolved)

MPKNYTLQNASNLGWLFYKDYYRQEPNVDFISTQGKESDTTADFFRKTNQRITAYQLNSESPLVAAFNNHFGTPLQLKTIYPGLITGSGLPHQTGSKGEFKLGFQFDYTTGLPYIPGSSIKGTLRSMFPFSLKDKGSTKRILPEYRKERMEYIRDLIIEVTNINEISDTEIQALEYAIFTNSTPSGKTIEFSLEEKDVFYDAFVADSKDGVMLSDDYITPHGENPLKDPKPILFLKIRPDVTINFYFKLCTTHLYKEKVCSSKQIEEIKKQNDFSSSDYKMITAHQKRNLFEKILLCIGIGAKTNIGYGQLKKL

MPKNYTLQNASNLGWLFYKDYYRQEPNVDFISTQGKESDTTADFFRKTNQRITAYQLNSESPLVAAFNNHFGTPLQLKTIYPGLITGSGLPHQTGSKGEFKLGFQFDYTTGLPYIPGSSIKGTLRSMFPFSLKDKGSTKRILPEYRKERMEYIRDLIIEVTNINEISDTEIQALEYAIFTNSTPSGKTIEFSLEEKDVFYDAFVADSKDGVMLSDDYITPHGENPLKDPKPILFLKIRPDVTINFYFKLCTTHLYKEKVCSSKQIEEIKKQNDFSSSDYKMITAHQKRNLFEKILLCIGIGAKTNIGYGQLKKL
RNA #6 49 bases

CACAAGGGACGCCACUUCAUGGAAAUAAAUCACUCACGAAUAGACACGA

CACAAGGGACGCCACUUCAUGGAAAUAAAUCACUCACGAAUAGACACGA
RNA #7 49 bases

AUUAAGACAUUCGUGAGUGAUUUAUUUCCAUGAAGUGGCGUCCCUUGUG

AUUAAGACAUUCGUGAGUGAUUUAUUUCCAUGAAGUGGCGUCCCUUGUG
Protein #8 600 residues (94% resolved)

MKYIAITLGPITRTIEMAESTKELWAASYFFSYLAKKIVEPFVKKNRTFQLPLINEEMQKPHCGAGLFPDRYIFKSEPGDLELLKQHSDQVLIEIAGHIASPSLPGTAKDVSQIYHYLKSYIKIYFIERTLESDDPHVVIPACEKYLNIIENQETFPEQEETMISHQKSDFLKFLITNVNGKIYRKDKNSIPRFTGSFLTRDAFGDMNGERLFESILEISASELNINIQQKALEVITANEKNKGEKYSDQIWDAEEIILNDNKAQLRPYHKYIAIIKSDGDSMGETIKSMGAYNIPITQLSKALLSFNIESINEIVAYGGKPIFIGGDDLLCFAPVCCNGNNVFNLVEKLSTCFDQCINQHLQQYINACSEAQRPLPSLSFGISITYHKYPMFEALHTTDYLLEMVAKDNLFKYTLSNKNILNENMKRFILKNKLAFSLQKHSGQIYHTAMSKKGKSYVKFNMLLQKYILKNKDMSKTQESEKFLSSVIQMIRAHAEILQIILQNEDKRTEMLKNYFDNNFNESCHLGYTGLFEDIQTLLCLRYQENIQDYQNRNEIIQQNTILTSDEKEILIVSPAMDAIHTIFTALQFIHFINYNKDE

MKYIAITLGPITRTIEMAESTKELWAASYFFSYLAKKIVEPFVKKNRTFQLPLINEEMQKPHCGAGLFPDRYIFKSEPGDLELLKQHSDQVLIEIAGHIASPSLPGTAKDVSQIYHYLKSYIKIYFIERTLESDDPHVVIPACEKYLNIIENQETFPEQEETMISHQKSDFLKFLITNVNGKIYRKDKNSIPRFTGSFLTRDAFGDMNGERLFESILEISASELNINIQQKALEVITANEKNKGEKYSDQIWDAEEIILNDNKAQLRPYHKYIAIIKSDGDSMGETIKSMGAYNIPITQLSKALLSFNIESINEIVAYGGKPIFIGGDDLLCFAPVCCNGNNVFNLVEKLSTCFDQCINQHLQQYINACSEAQRPLPSLSFGISITYHKYPMFEALHTTDYLLEMVAKDNLFKYTLSNKNILNENMKRFILKNKLAFSLQKHSGQIYHTAMSKKGKSYVKFNMLLQKYILKNKDMSKTQESEKFLSSVIQMIRAHAEILQIILQNEDKRTEMLKNYFDNNFNESCHLGYTGLFEDIQTLLCLRYQENIQDYQNRNEIIQQNTILTSDEKEILIVSPAMDAIHTIFTALQFIHFINYNKDE
Non polymer #9 ATP

InChI=1S/C10H16N5O13P3/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1

c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@@](=O)(O)O[P@](=O)(O)OP(=O)(O)O)O)O)N
Non polymer #10 MN

InChI=1S/Mn/q+2

[Mn+2]
Non polymer #11 SAM

InChI=1S/C15H22N6O5S/c1-27(3-2-7(16)15(24)25)4-8-10(22)11(23)14(26-8)21-6-20-9-12(17)18-5-19-13(9)21/h5-8,10-11,14,22-23H,2-4,16H2,1H3,(H2-,17,18,19,24,25)/t7-,8+,10+,11+,14+,27-/m0/s1

C[S@@+](CC[C@@H](C(=O)[O-])N)C[C@@H]1[C@H]([C@H]([C@@H](O1)n2cnc3c2ncnc3N)O)O
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Reference