2025-10-18_00000346|hard|9OM4

Cryo-EM structure of filament form Acidithiobacillus caldus (Aca) short prokaryotic argonautes, HNH-associated (SPARHA) with gRNA and tDNA; Electron microscopy 3.38Å
Protein #1 435 residues (63% resolved)

MAIEPRTKQILWGRAGATCSFPGCGRNLVRDATPEDREVLVGEIAHIVGQSKGGPRAEMSVPGGNVDGYDNLILLCHEHHELVDQQPNTYPVEKLRQYKTDHEEWVRARLSREQEFEGLTRPEAMVTETVYSNVLPITQLPHYVYSGPCTVPEAEVKTLIKWPSDKRILTPYIIRGGRLYAFNDLKDYESPFATIVDPYSAQRHTLNEWLDKPDEMRWYVELLNRTVNKITGHLRLKLDKEHHRYFFEPDEPGKDKSVTYQSVGGVRSERKVAWNPHFKHNDMPKRYWEHLAVGLRFHRLGDMAWGFAIRPERRFTKDGFESLEGKATGKKSTKKKSRMYNFDVLKEVQFWRDFLSQGNPRITCLFGKQALVIDNSLMSAAITWPEISGDQANRMSASYEDDLFSLADLQEVSEFDEFDSDTDDLETEDEVQGED

MAIEPRTKQILWGRAGATCSFPGCGRNLVRDATPEDREVLVGEIAHIVGQSKGGPRAEMSVPGGNVDGYDNLILLCHEHHELVDQQPNTYPVEKLRQYKTDHEEWVRARLSREQEFEGLTRPEAMVTETVYSNVLPITQLPHYVYSGPCTVPEAEVKTLIKWPSDKRILTPYIIRGGRLYAFNDLKDYESPFATIVDPYSAQRHTLNEWLDKPDEMRWYVELLNRTVNKITGHLRLKLDKEHHRYFFEPDEPGKDKSVTYQSVGGVRSERKVAWNPHFKHNDMPKRYWEHLAVGLRFHRLGDMAWGFAIRPERRFTKDGFESLEGKATGKKSTKKKSRMYNFDVLKEVQFWRDFLSQGNPRITCLFGKQALVIDNSLMSAAITWPEISGDQANRMSASYEDDLFSLADLQEVSEFDEFDSDTDDLETEDEVQGED
Protein #2 471 residues (100% resolved)

MKTNTATVRCELFEEPSLEFASSRLHLCPKRGITIFGPRSLDMGKRHPDIVRVGFIGTGETIDSATRWLESCADGVEGDVANYRFPGFRADRGFFSDLSFDAETVERLTLRELEALARPRTKRERFEQAVALLDDKLRLLSQKDRPPDYVVLALPTELVKAQGRVDYYDKQDGEVHRDLRRAIKASAMKYRLPTQILLQRTTEATPGSKDVDHLSKCAWNFFTGLYYKTGGVPWIPAGLSNGTCYVGISFHQLLGSKNSGYFTGLAQAFDEQGNGLVLRGQDFVWDVGKHGNSAHMPAPIAEELVSRVLKRYRDELKQSPRRVVIHKTTEFWPEERAGLQSALSGVDQFDLVAVRPTEDIRLFRDARYPILRGSHISLGDMHLLYTTGYISALQAFPHGHVPLPLKLTDHLGDSSISTVLRELMVLTKMNWNSANFGGLLPITLRFSKLVGEIMKEIPNSVDPLPQFKYYM

MKTNTATVRCELFEEPSLEFASSRLHLCPKRGITIFGPRSLDMGKRHPDIVRVGFIGTGETIDSATRWLESCADGVEGDVANYRFPGFRADRGFFSDLSFDAETVERLTLRELEALARPRTKRERFEQAVALLDDKLRLLSQKDRPPDYVVLALPTELVKAQGRVDYYDKQDGEVHRDLRRAIKASAMKYRLPTQILLQRTTEATPGSKDVDHLSKCAWNFFTGLYYKTGGVPWIPAGLSNGTCYVGISFHQLLGSKNSGYFTGLAQAFDEQGNGLVLRGQDFVWDVGKHGNSAHMPAPIAEELVSRVLKRYRDELKQSPRRVVIHKTTEFWPEERAGLQSALSGVDQFDLVAVRPTEDIRLFRDARYPILRGSHISLGDMHLLYTTGYISALQAFPHGHVPLPLKLTDHLGDSSISTVLRELMVLTKMNWNSANFGGLLPITLRFSKLVGEIMKEIPNSVDPLPQFKYYM
RNA #3 18 bases

AUUUGGUUGCGGUGCUCU

AUUUGGUUGCGGUGCUCU
DNA #4 18 bases

(DA)(DG)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DA)(DA)(DC)(DC)(DA)(DA)(DA)(DT)

AGAGCACCGCAACCAAAT
Non polymer #5 MN

InChI=1S/Mn/q+2

[Mn+2]
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Reference