2025-09-13_00000100|hard|9JWN

Cryo-EM structure of FrCas9 in complex with sgRNA and 43-bp dsDNA substrate; Electron microscopy 2.89Å
DNA #1 13 bases

(DT)(DG)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DT)(DG)(DC)(DA)

TGTGTATATTGCA
RNA #2 125 bases

UGCAAGACAUAGAAAUAACCUGGUUUGAGUGUCUUGUUAAUUGAAAAAUUAACAAGAUGAGUUCAAAUCAGGCUCCUAGAGAGAUCCGAACUUACCUUCAUGGCGGGCAUUGUGCCCUUUUUUUU

UGCAAGACAUAGAAAUAACCUGGUUUGAGUGUCUUGUUAAUUGAAAAAUUAACAAGAUGAGUUCAAAUCAGGCUCCUAGAGAGAUCCGAACUUACCUUCAUGGCGGGCAUUGUGCCCUUUUUUUU
DNA #3 43 bases

(DT)(DG)(DC)(DA)(DA)(DT)(DA)(DT)(DA)(DC)(DA)(DC)(DA)(DG)(DG)(DT)(DT)(DA)(DT)(DT)(DT)(DC)(DT)(DA)(DT)(DG)(DT)(DC)(DT)(DT)(DG)(DC)(DA)(DG)(DT)(DG)(DA)(DA)(DG)(DT)(DG)(DT)(DT)

TGCAATATACACAGGTTATTTCTATGTCTTGCAGTGAAGTGTT
Protein #4 1372 residues (77% resolved)

MCTKESEKLNKNADYYIGLDMGTSSAGWAVSDSEYNLIRRKGKDLWGVRQFEEAKTAAERRGFRVARRRKQRQQVRNRLLSEEFQNEITKIDSGFLKRMEDSRFVISDKRVPEKYTLFNDSGYTDVEYYNQYPTIYHLRKALIESNERFDIRLVFLGIHSLFQHPGHFLDKGDVDTDNTGPEELIQFLEDCMNEIQISIPLVSNQKVLTDILTDSRITRRDKEQQILEILQPNKESKKAVSQFVKVLTGQKAKLGDLIMMEDKDTEEYKYSFSFREKTLEEILPDIEGVIDGLALEYIESIYSLYSWSLLNSYMKDTLTGHYYSYLAEARVAAYDKHHSDLVKLKTLFREYIPEEYDNFFRKMEKANYSHYIGSTEYDGEKRCRTAKAKQEDFYKSINKMLEKIPECSEKTEIQKEIIEGTFLLKQTGPQNGFVPNQLQLKELRKILQNASKHYPFLTEKDERDMTAIDRIEALFSFRIPYYIGPLKNTDNQGHGWAVRRDGHEQIPVRPWNFEEIIDESASADLFIKNLVNSCTYLRTEKVLPKSSLLYQEFEVLNELNNLRINGMYPDEIQPGLKRMIFEQCFYSGKKVTGKKLQLFLRSVLTNSSTEEFVLTGIDKDFKSSLSSYKKFCELFGVKTLNDTQKVMAEQIIEWSTVYGDSRKFLKRKLEDNYPELTDQQIRRIAGFKFSEWGNLSRAFLEMEGYKDEAGNPVTIIRALRDTQKNLMQLLSNDSAFAKKLQELNDYVTRDIWSIEPDDLDGMYLSAPVRRMIWQTFLILREVVDTIGYSPKKIFMEMARGEQEKKRTASRKKQLIDLYKEAGMKNDELFGDLESLEEAQLRSKKLYLYFRQMGRDIYSGKLIDFMDVLHGNRYDIDAIHPQSKKKDDSLENNLVLTSKDFNNHIKQDVYPIPEQIQSRQKGFWAMLLKQGFMSQEKYNRLMRTTPFTDEELAEFVNRQLVETRQGTKAIISLINQCFPDSEVVYVKAGNTSDFRQRFDIPKSRDLNNYHHAVDAYLNIVVGNVYDTKFTKNPINFIKKMRKSGNLHSYSLRRMYDFNVQRGDQTAWVAENDTTLKTVKKTAFKTSPMVTKRTYERKGGLADSVLIAAKKAKPGVHLPVKTSDSRFANQVSTYGGYDNVKGSHFFLVEHQQKKKTIRSIENVPIHLKEKLKTKEELEHYCAQVLGMVQPDVRLTRIPMYSLLLIDGYYYYLTGRTGGNLSLSNAVELCLPAKEQAHIRMISKIAGGRSTDALSAEAKDDFRKKNLRLYDELAEKHRSTIFSKRKNPIGPKLLKYREAFVKQTIENQCKVILQILKLTSTNCKTSADLKLIGGSGQEGVMSISKLLRAEKYAEFYLICQSPSGIYETRKNLLTI

MCTKESEKLNKNADYYIGLDMGTSSAGWAVSDSEYNLIRRKGKDLWGVRQFEEAKTAAERRGFRVARRRKQRQQVRNRLLSEEFQNEITKIDSGFLKRMEDSRFVISDKRVPEKYTLFNDSGYTDVEYYNQYPTIYHLRKALIESNERFDIRLVFLGIHSLFQHPGHFLDKGDVDTDNTGPEELIQFLEDCMNEIQISIPLVSNQKVLTDILTDSRITRRDKEQQILEILQPNKESKKAVSQFVKVLTGQKAKLGDLIMMEDKDTEEYKYSFSFREKTLEEILPDIEGVIDGLALEYIESIYSLYSWSLLNSYMKDTLTGHYYSYLAEARVAAYDKHHSDLVKLKTLFREYIPEEYDNFFRKMEKANYSHYIGSTEYDGEKRCRTAKAKQEDFYKSINKMLEKIPECSEKTEIQKEIIEGTFLLKQTGPQNGFVPNQLQLKELRKILQNASKHYPFLTEKDERDMTAIDRIEALFSFRIPYYIGPLKNTDNQGHGWAVRRDGHEQIPVRPWNFEEIIDESASADLFIKNLVNSCTYLRTEKVLPKSSLLYQEFEVLNELNNLRINGMYPDEIQPGLKRMIFEQCFYSGKKVTGKKLQLFLRSVLTNSSTEEFVLTGIDKDFKSSLSSYKKFCELFGVKTLNDTQKVMAEQIIEWSTVYGDSRKFLKRKLEDNYPELTDQQIRRIAGFKFSEWGNLSRAFLEMEGYKDEAGNPVTIIRALRDTQKNLMQLLSNDSAFAKKLQELNDYVTRDIWSIEPDDLDGMYLSAPVRRMIWQTFLILREVVDTIGYSPKKIFMEMARGEQEKKRTASRKKQLIDLYKEAGMKNDELFGDLESLEEAQLRSKKLYLYFRQMGRDIYSGKLIDFMDVLHGNRYDIDAIHPQSKKKDDSLENNLVLTSKDFNNHIKQDVYPIPEQIQSRQKGFWAMLLKQGFMSQEKYNRLMRTTPFTDEELAEFVNRQLVETRQGTKAIISLINQCFPDSEVVYVKAGNTSDFRQRFDIPKSRDLNNYHHAVDAYLNIVVGNVYDTKFTKNPINFIKKMRKSGNLHSYSLRRMYDFNVQRGDQTAWVAENDTTLKTVKKTAFKTSPMVTKRTYERKGGLADSVLIAAKKAKPGVHLPVKTSDSRFANQVSTYGGYDNVKGSHFFLVEHQQKKKTIRSIENVPIHLKEKLKTKEELEHYCAQVLGMVQPDVRLTRIPMYSLLLIDGYYYYLTGRTGGNLSLSNAVELCLPAKEQAHIRMISKIAGGRSTDALSAEAKDDFRKKNLRLYDELAEKHRSTIFSKRKNPIGPKLLKYREAFVKQTIENQCKVILQILKLTSTNCKTSADLKLIGGSGQEGVMSISKLLRAEKYAEFYLICQSPSGIYETRKNLLTI
Download coordinates
Reference