2025-09-13_00000099|hard|9JWK

Cryo-EM structure of FrCas9 in complex with sgRNA and 26-nt TS and 4-nt NTS substrates; Electron microscopy 3.46Å
Protein #1 1372 residues (85% resolved)

MCTKESEKLNKNADYYIGLDMGTSSAGWAVSDSEYNLIRRKGKDLWGVRQFEEAKTAAERRGFRVARRRKQRQQVRNRLLSEEFQNEITKIDSGFLKRMEDSRFVISDKRVPEKYTLFNDSGYTDVEYYNQYPTIYHLRKALIESNERFDIRLVFLGIHSLFQHPGHFLDKGDVDTDNTGPEELIQFLEDCMNEIQISIPLVSNQKVLTDILTDSRITRRDKEQQILEILQPNKESKKAVSQFVKVLTGQKAKLGDLIMMEDKDTEEYKYSFSFREKTLEEILPDIEGVIDGLALEYIESIYSLYSWSLLNSYMKDTLTGHYYSYLAEARVAAYDKHHSDLVKLKTLFREYIPEEYDNFFRKMEKANYSHYIGSTEYDGEKRCRTAKAKQEDFYKSINKMLEKIPECSEKTEIQKEIIEGTFLLKQTGPQNGFVPNQLQLKELRKILQNASKHYPFLTEKDERDMTAIDRIEALFSFRIPYYIGPLKNTDNQGHGWAVRRDGHEQIPVRPWNFEEIIDESASADLFIKNLVNSCTYLRTEKVLPKSSLLYQEFEVLNELNNLRINGMYPDEIQPGLKRMIFEQCFYSGKKVTGKKLQLFLRSVLTNSSTEEFVLTGIDKDFKSSLSSYKKFCELFGVKTLNDTQKVMAEQIIEWSTVYGDSRKFLKRKLEDNYPELTDQQIRRIAGFKFSEWGNLSRAFLEMEGYKDEAGNPVTIIRALRDTQKNLMQLLSNDSAFAKKLQELNDYVTRDIWSIEPDDLDGMYLSAPVRRMIWQTFLILREVVDTIGYSPKKIFMEMARGEQEKKRTASRKKQLIDLYKEAGMKNDELFGDLESLEEAQLRSKKLYLYFRQMGRDIYSGKLIDFMDVLHGNRYDIDAIHPQSKKKDDSLENNLVLTSKDFNNHIKQDVYPIPEQIQSRQKGFWAMLLKQGFMSQEKYNRLMRTTPFTDEELAEFVNRQLVETRQGTKAIISLINQCFPDSEVVYVKAGNTSDFRQRFDIPKSRDLNNYHHAVDAYLNIVVGNVYDTKFTKNPINFIKKMRKSGNLHSYSLRRMYDFNVQRGDQTAWVAENDTTLKTVKKTAFKTSPMVTKRTYERKGGLADSVLIAAKKAKPGVHLPVKTSDSRFANQVSTYGGYDNVKGSHFFLVEHQQKKKTIRSIENVPIHLKEKLKTKEELEHYCAQVLGMVQPDVRLTRIPMYSLLLIDGYYYYLTGRTGGNLSLSNAVELCLPAKEQAHIRMISKIAGGRSTDALSAEAKDDFRKKNLRLYDELAEKHRSTIFSKRKNPIGPKLLKYREAFVKQTIENQCKVILQILKLTSTNCKTSADLKLIGGSGQEGVMSISKLLRAEKYAEFYLICQSPSGIYETRKNLLTI

MCTKESEKLNKNADYYIGLDMGTSSAGWAVSDSEYNLIRRKGKDLWGVRQFEEAKTAAERRGFRVARRRKQRQQVRNRLLSEEFQNEITKIDSGFLKRMEDSRFVISDKRVPEKYTLFNDSGYTDVEYYNQYPTIYHLRKALIESNERFDIRLVFLGIHSLFQHPGHFLDKGDVDTDNTGPEELIQFLEDCMNEIQISIPLVSNQKVLTDILTDSRITRRDKEQQILEILQPNKESKKAVSQFVKVLTGQKAKLGDLIMMEDKDTEEYKYSFSFREKTLEEILPDIEGVIDGLALEYIESIYSLYSWSLLNSYMKDTLTGHYYSYLAEARVAAYDKHHSDLVKLKTLFREYIPEEYDNFFRKMEKANYSHYIGSTEYDGEKRCRTAKAKQEDFYKSINKMLEKIPECSEKTEIQKEIIEGTFLLKQTGPQNGFVPNQLQLKELRKILQNASKHYPFLTEKDERDMTAIDRIEALFSFRIPYYIGPLKNTDNQGHGWAVRRDGHEQIPVRPWNFEEIIDESASADLFIKNLVNSCTYLRTEKVLPKSSLLYQEFEVLNELNNLRINGMYPDEIQPGLKRMIFEQCFYSGKKVTGKKLQLFLRSVLTNSSTEEFVLTGIDKDFKSSLSSYKKFCELFGVKTLNDTQKVMAEQIIEWSTVYGDSRKFLKRKLEDNYPELTDQQIRRIAGFKFSEWGNLSRAFLEMEGYKDEAGNPVTIIRALRDTQKNLMQLLSNDSAFAKKLQELNDYVTRDIWSIEPDDLDGMYLSAPVRRMIWQTFLILREVVDTIGYSPKKIFMEMARGEQEKKRTASRKKQLIDLYKEAGMKNDELFGDLESLEEAQLRSKKLYLYFRQMGRDIYSGKLIDFMDVLHGNRYDIDAIHPQSKKKDDSLENNLVLTSKDFNNHIKQDVYPIPEQIQSRQKGFWAMLLKQGFMSQEKYNRLMRTTPFTDEELAEFVNRQLVETRQGTKAIISLINQCFPDSEVVYVKAGNTSDFRQRFDIPKSRDLNNYHHAVDAYLNIVVGNVYDTKFTKNPINFIKKMRKSGNLHSYSLRRMYDFNVQRGDQTAWVAENDTTLKTVKKTAFKTSPMVTKRTYERKGGLADSVLIAAKKAKPGVHLPVKTSDSRFANQVSTYGGYDNVKGSHFFLVEHQQKKKTIRSIENVPIHLKEKLKTKEELEHYCAQVLGMVQPDVRLTRIPMYSLLLIDGYYYYLTGRTGGNLSLSNAVELCLPAKEQAHIRMISKIAGGRSTDALSAEAKDDFRKKNLRLYDELAEKHRSTIFSKRKNPIGPKLLKYREAFVKQTIENQCKVILQILKLTSTNCKTSADLKLIGGSGQEGVMSISKLLRAEKYAEFYLICQSPSGIYETRKNLLTI
RNA #2 125 bases

UGCAAGACAUAGAAAUAACCUGGUUUGAGUGUCUUGUUAAUUGAAAAAUUAACAAGAUGAGUUCAAAUCAGGCUCCUAGAGAGAUCCGAACUUACCUUCAUGGCGGGCAUUGUGCCCUUUUUUUU

UGCAAGACAUAGAAAUAACCUGGUUUGAGUGUCUUGUUAAUUGAAAAAUUAACAAGAUGAGUUCAAAUCAGGCUCCUAGAGAGAUCCGAACUUACCUUCAUGGCGGGCAUUGUGCCCUUUUUUUU
DNA #3 26 bases

(DT)(DA)(DC)(DA)(DC)(DA)(DG)(DG)(DT)(DT)(DA)(DT)(DT)(DT)(DC)(DT)(DA)(DT)(DG)(DT)(DC)(DT)(DT)(DG)(DC)(DA)

TACACAGGTTATTTCTATGTCTTGCA
Download coordinates
Reference