2025-09-06_00000304|hard|9XY9

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence; X-ray diffraction 2.00Å
Protein #1 300 residues (98% resolved)

MASSRRESINPWILTGFADAEGSFLLRIRNNNKSSVGYSTELGFQITLHNKDKSILENIQSTWKVGVIANSGDNAVSLKVTRFEDLKVIIDHFEKYPLITQKLGDYKLFKQAFSVMENKEHLKENGIKELVRIKAKLNWGLTDELKKAFPENISKERSLINKNIPNFKWLAGFTSGEGHFRVNLIKSKSKLGVQVQLVFTITQHIKDKNLMNSLITYLGCGYIYEFNKSEFSWLDFRVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGR

MASSRRESINPWILTGFADAEGSFLLRIRNNNKSSVGYSTELGFQITLHNKDKSILENIQSTWKVGVIANSGDNAVSLKVTRFEDLKVIIDHFEKYPLITQKLGDYKLFKQAFSVMENKEHLKENGIKELVRIKAKLNWGLTDELKKAFPENISKERSLINKNIPNFKWLAGFTSGEGHFRVNLIKSKSKLGVQVQLVFTITQHIKDKNLMNSLITYLGCGYIYEFNKSEFSWLDFRVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGR
DNA #2 26 bases

(DG)(DG)(DG)(DT)(DT)(DT)(DC)(DC)(DA)(DC)(DT)(DT)(DA)(DT)(DT)(DC)(DA)(DA)(DC)(DA)(DT)(DT)(DT)(DT)(DA)(DG)

GGGTTTCCACTTATTCAACATTTTAG
DNA #3 26 bases

(DC)(DC)(DT)(DA)(DA)(DA)(DA)(DT)(DG)(DT)(DT)(DG)(DA)(DA)(DT)(DA)(DA)(DG)(DT)(DG)(DG)(DA)(DA)(DA)(DC)(DC)

CCTAAAATGTTGAATAAGTGGAAACC
Non polymer #4 CA

InChI=1S/Ca/q+2

[Ca+2]
Non polymer #5 EDO

InChI=1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

C(CO)O
Non polymer #6 NA

InChI=1S/Na/q+1

[Na+]
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Reference