2025-09-06_00000226|hard|9PZ7

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence; X-ray diffraction 2.50Å
Protein #1 300 residues (98% resolved)

MASSRRESINPWILTGFADAEGSFLLRIRNNNKSSVGYSTELGFQITLHNKDKSILENIQSTWKVGVIANSGDNAVSLKVTRFEDLKVIIDHFEKYPLITQKLGDYKLFKQAFSVMENKEHLKENGIKELVRIKAKLNWGLTDELKKAFPENISKERSLINKNIPNFKWLAGFTSGEGSFYVNLIKSKSKLGVQVQLRFDITQHIKDKNLMNSLITYLGCGYIIEYNKSEFSWLNFRVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGR

MASSRRESINPWILTGFADAEGSFLLRIRNNNKSSVGYSTELGFQITLHNKDKSILENIQSTWKVGVIANSGDNAVSLKVTRFEDLKVIIDHFEKYPLITQKLGDYKLFKQAFSVMENKEHLKENGIKELVRIKAKLNWGLTDELKKAFPENISKERSLINKNIPNFKWLAGFTSGEGSFYVNLIKSKSKLGVQVQLRFDITQHIKDKNLMNSLITYLGCGYIIEYNKSEFSWLNFRVTKFSDINDKIIPVFQENTLIGVKLEDFEDWCKVAKLIEEKKHLTESGLDEIKKIKLNMNKGR
DNA #2 26 bases

(DG)(DG)(DG)(DT)(DT)(DT)(DC)(DC)(DA)(DC)(DT)(DT)(DA)(DT)(DT)(DC)(DA)(DT)(DC)(DA)(DT)(DT)(DT)(DT)(DA)(DG)

GGGTTTCCACTTATTCATCATTTTAG
DNA #3 26 bases

(DC)(DC)(DT)(DA)(DA)(DA)(DA)(DT)(DG)(DA)(DT)(DG)(DA)(DA)(DT)(DA)(DA)(DG)(DT)(DG)(DG)(DA)(DA)(DA)(DC)(DC)

CCTAAAATGATGAATAAGTGGAAACC
Non polymer #4 CA

InChI=1S/Ca/q+2

[Ca+2]
Non polymer #5 CL

InChI=1S/ClH/h1H/p-1

[Cl-]
Download coordinates
Reference