2025-08-16_00000306|hard|9LGI

Cryo-EM structure of a type II-D CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA; Electron microscopy 2.86Å
Protein #1 762 residues (90% resolved)

MEKELVLGIDYGGKYTGLAVVNQKNNQVLYARTVKMRDDVTDILAGRREQRSLRRTLQTKKKRLRELKNYLESIGGIYEESSGTFTIEPFRTVYSLAHKRGYDYADLPEEKTSEEIEAMDAKERKQWEKEKKELEETQRNSRHRDEVLRDVRNVMTEGNLSEEQIIKVESIFNKQYRHKRFNNRILTKCKVCGKNTPLRINVRELLLENIVRYLPLQNKERELLKLTILKGHQQDINEIFKHFRKVYKITLNQKDWPGKNLIDIARNQLRGRLLFCKVHFPENEKYVSIEKKTFRLAPSLKTKIENVLSVIKDDILPNFTLNNVVMESNNFDIAAKTKGKKRLLKEEYSKGHRESGETRKEALLRETDSRCIYCGKGIDLSNAHEDHIFPRKAGGINIFGNLVACCSVCNEEKRGRTPLESGILPKPEIVSFITNDLKKKILEDAQYINTLDFNKYMSHASIGWRHMRDRLRELTGNKELLIKRQSGIYTAYFRKWWGFIKERGNHGHHALDAVILASKKSYAEDGKVDMTIKPCGEDGKEFDIERHLSEMKEFRRDKGGKSAPLHDRNPLSFKNDIITRRFMVTEIECGKEAVIISEEYRKKLTEAFKRFGIAKGKYLTDEQAKDAGFYLRKNGEGVMSLKCEVKGTGYNQMIRIKNNIFKTNVHNVGVAVFLDEKGKKRACELKNPRLSKHFVKPAEQVKGKVIFILKRGNMVTVEGEEMIYRVKKLGTSPVIEAIVGSDGKTRTVSATKLLKINHTKKV

MEKELVLGIDYGGKYTGLAVVNQKNNQVLYARTVKMRDDVTDILAGRREQRSLRRTLQTKKKRLRELKNYLESIGGIYEESSGTFTIEPFRTVYSLAHKRGYDYADLPEEKTSEEIEAMDAKERKQWEKEKKELEETQRNSRHRDEVLRDVRNVMTEGNLSEEQIIKVESIFNKQYRHKRFNNRILTKCKVCGKNTPLRINVRELLLENIVRYLPLQNKERELLKLTILKGHQQDINEIFKHFRKVYKITLNQKDWPGKNLIDIARNQLRGRLLFCKVHFPENEKYVSIEKKTFRLAPSLKTKIENVLSVIKDDILPNFTLNNVVMESNNFDIAAKTKGKKRLLKEEYSKGHRESGETRKEALLRETDSRCIYCGKGIDLSNAHEDHIFPRKAGGINIFGNLVACCSVCNEEKRGRTPLESGILPKPEIVSFITNDLKKKILEDAQYINTLDFNKYMSHASIGWRHMRDRLRELTGNKELLIKRQSGIYTAYFRKWWGFIKERGNHGHHALDAVILASKKSYAEDGKVDMTIKPCGEDGKEFDIERHLSEMKEFRRDKGGKSAPLHDRNPLSFKNDIITRRFMVTEIECGKEAVIISEEYRKKLTEAFKRFGIAKGKYLTDEQAKDAGFYLRKNGEGVMSLKCEVKGTGYNQMIRIKNNIFKTNVHNVGVAVFLDEKGKKRACELKNPRLSKHFVKPAEQVKGKVIFILKRGNMVTVEGEEMIYRVKKLGTSPVIEAIVGSDGKTRTVSATKLLKINHTKKV
RNA #2 156 bases

GGUUAUAACUGAUAUAUGGGGUUACAGUUAAGGCUCUUUGAAAAAAGAGCCUUAAUUGUAAAACGCCUAUACGGUGAGGGUAUGUACGUUUGGGUUUGUCCAGCCUAAACCUCUACGCCAGAAAUGGCACCUUCAUUGUGGGUUAGGACAAUUUAA

GGUUAUAACUGAUAUAUGGGGUUACAGUUAAGGCUCUUUGAAAAAAGAGCCUUAAUUGUAAAACGCCUAUACGGUGAGGGUAUGUACGUUUGGGUUUGUCCAGCCUAAACCUCUACGCCAGAAAUGGCACCUUCAUUGUGGGUUAGGACAAUUUAA
DNA #3 41 bases

(DT)(DC)(DT)(DA)(DG)(DA)(DG)(DG)(DA)(DT)(DC)(DC)(DC)(DC)(DC)(DC)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DA)(DG)(DT)(DT)(DA)(DT)(DA)(DA)(DC)(DC)(DG)(DA)(DA)(DT)(DT)(DC)(DA)(DC)

TCTAGAGGATCCCCCCATATATCAGTTATAACCGAATTCAC
DNA #4 41 bases

(DG)(DT)(DG)(DA)(DA)(DT)(DT)(DC)(DC)(DC)(DA)(DA)(DT)(DA)(DT)(DT)(DG)(DA)(DC)(DT)(DA)(DT)(DA)(DT)(DA)(DC)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DC)(DC)(DT)(DC)(DT)(DA)(DG)(DA)

GTGAATTCCCAATATTGACTATATACCCGGGATCCTCTAGA
Non polymer #5 ZN

InChI=1S/Zn/q+2

[Zn+2]
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Reference