2025-07-26_00000334|hard|9UA2

Cryo-EM structure of PsCas9-sgRNA-dsDNA (30 nt) ternary complex; Electron microscopy 2.83Å
Protein #1 1435 residues (98% resolved)

HHHHHHVAGAGAGENLYFQGMTVKSTLAVDMGGRYTGIFSYTTDSGFPKAKEARAYVLNMPDNDALTYSMAARTQTRHRIRSQQRFVLARRLTYILIEGKLKRKLSPREKEAISSLLRRRGYSRLESELDLSVLQGVESGFFKCFLPNFDEDENLLTQWTSLTDGYLQNNSDSRRQIQIFLESSKDSKEFLTVVKSQHQDTKEYKNALKVMRDDAESMIEQSMFGHKHRRLYLEAIAQDIPRDSRLKPIIEAFSGVEKFHHFIGNLSNLQLRALRWYFNDPSMKNNVFDKERLKSVLVRAYQFFHYPKDLTQQRAEVLNAYEGATDILETLQTLNPELTIPPYEDQNNRRPPLDQTLWLSPRLLDQRYGDTWEIWVQNLLRSPLSKGIDENLDTILITTDRKARLLERQSGRLIHYTSQKLYHSYVLQRLLDRTVENDAYLLKTLVSSNRGNSNEIHQAQERLTRDLGSQHIKKFLDFVRQYYDEVDKAKRGLWFIVEKPLMERADIHPPMKNDSVILRLVGNILCVSDLVDLSFWTRKVKGQSTVRSLCTAIEKTRKEYGNSFNYLYQRALYLQSKGKKLSAEDKDFIKLQSNVLLVSDVIAEALDIKEEQKKKFANPFSLAQLYNIIETEKSGFISTTLAAVDENAWRNNLQGKARCVQLCADTVRPFDGALRNILDRQAYEIAKLKAEELLSTELKNQTIDLVVLLESNQFAFSASLAEVKKSANTAAIRQKVAKAQKRQQDRWLSKDERIKSASRGLCPYTGKNLGDKGEVDHIIPRSLSMNYMGSILNSEANLIYCSQEGAQLKLNGRKKLSDLADNYLKVVFGTADRGTICKYIEKSVSELTDAKIVQFELLDRSQQDAVRHALFLEDFSEARRRIIRLLGKINTARVNGTQAWFAKSFITKLRELTKEWCANNQITLAFDLYRLDAQTVSQDYRKKFALINKDWAKPDDKKQPIASHAIDAFCVFAAAKDKRNIANVLGVFDEVAEEQNLKTIAQLMPSEVNLISPKRKSILDKNEVGSRALMKEGIFAEHFLPILVRGDDCRIGFDWSESGSVKVKDADKLFGVLDGLLKQSQKRSVNGFETYTVDRIKAFELLHDVFIRPCSQKMLEQAEVLEKLHYITQNISVTSVYDAVNRQFKCREEILKDKDFDIKVDLGNRFGSAKGKITLPAKREWEKLVNRSELKNLIKDKLSDKGSEKTPDGETLIYDIFRSIPVQKLSHKATRRVWSLPKIPSISSGVRIKRKDSNGNDIYQLYMLNDTKCKGFVVNEKGVIDWSSDLVADLYKQPTLTILNGRYLKADQYVRMDQWYEVDCGRDDVIVKMCPGTSGRRYIEITQSKKQFEDWTGYISGSFWNYPVTIKLSSQQIANFVKNSQMPLLGKPRSGQITVITLGNTLKYWYCVESKNSMMNEAYQKAYLVHFNQKRPAAT

HHHHHHVAGAGAGENLYFQGMTVKSTLAVDMGGRYTGIFSYTTDSGFPKAKEARAYVLNMPDNDALTYSMAARTQTRHRIRSQQRFVLARRLTYILIEGKLKRKLSPREKEAISSLLRRRGYSRLESELDLSVLQGVESGFFKCFLPNFDEDENLLTQWTSLTDGYLQNNSDSRRQIQIFLESSKDSKEFLTVVKSQHQDTKEYKNALKVMRDDAESMIEQSMFGHKHRRLYLEAIAQDIPRDSRLKPIIEAFSGVEKFHHFIGNLSNLQLRALRWYFNDPSMKNNVFDKERLKSVLVRAYQFFHYPKDLTQQRAEVLNAYEGATDILETLQTLNPELTIPPYEDQNNRRPPLDQTLWLSPRLLDQRYGDTWEIWVQNLLRSPLSKGIDENLDTILITTDRKARLLERQSGRLIHYTSQKLYHSYVLQRLLDRTVENDAYLLKTLVSSNRGNSNEIHQAQERLTRDLGSQHIKKFLDFVRQYYDEVDKAKRGLWFIVEKPLMERADIHPPMKNDSVILRLVGNILCVSDLVDLSFWTRKVKGQSTVRSLCTAIEKTRKEYGNSFNYLYQRALYLQSKGKKLSAEDKDFIKLQSNVLLVSDVIAEALDIKEEQKKKFANPFSLAQLYNIIETEKSGFISTTLAAVDENAWRNNLQGKARCVQLCADTVRPFDGALRNILDRQAYEIAKLKAEELLSTELKNQTIDLVVLLESNQFAFSASLAEVKKSANTAAIRQKVAKAQKRQQDRWLSKDERIKSASRGLCPYTGKNLGDKGEVDHIIPRSLSMNYMGSILNSEANLIYCSQEGAQLKLNGRKKLSDLADNYLKVVFGTADRGTICKYIEKSVSELTDAKIVQFELLDRSQQDAVRHALFLEDFSEARRRIIRLLGKINTARVNGTQAWFAKSFITKLRELTKEWCANNQITLAFDLYRLDAQTVSQDYRKKFALINKDWAKPDDKKQPIASHAIDAFCVFAAAKDKRNIANVLGVFDEVAEEQNLKTIAQLMPSEVNLISPKRKSILDKNEVGSRALMKEGIFAEHFLPILVRGDDCRIGFDWSESGSVKVKDADKLFGVLDGLLKQSQKRSVNGFETYTVDRIKAFELLHDVFIRPCSQKMLEQAEVLEKLHYITQNISVTSVYDAVNRQFKCREEILKDKDFDIKVDLGNRFGSAKGKITLPAKREWEKLVNRSELKNLIKDKLSDKGSEKTPDGETLIYDIFRSIPVQKLSHKATRRVWSLPKIPSISSGVRIKRKDSNGNDIYQLYMLNDTKCKGFVVNEKGVIDWSSDLVADLYKQPTLTILNGRYLKADQYVRMDQWYEVDCGRDDVIVKMCPGTSGRRYIEITQSKKQFEDWTGYISGSFWNYPVTIKLSSQQIANFVKNSQMPLLGKPRSGQITVITLGNTLKYWYCVESKNSMMNEAYQKAYLVHFNQKRPAAT
RNA #2 131 bases

GUGGGGCCACUAGGGACAGGAUGUUUCAGUUAUUCGUGAAAACGAAUGAAGUCACUCUUAAAGUGAGCUGAAAUCACUAAAAAUUAAGAUUGAACCCGGCUACUGACUCUGUCAUCCGGGUUUACUUAUUU

GUGGGGCCACUAGGGACAGGAUGUUUCAGUUAUUCGUGAAAACGAAUGAAGUCACUCUUAAAGUGAGCUGAAAUCACUAAAAAUUAAGAUUGAACCCGGCUACUGACUCUGUCAUCCGGGUUUACUUAUUU
DNA #3 30 bases

(DG)(DT)(DG)(DG)(DG)(DG)(DC)(DC)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DG)(DA)(DT)(DT)(DG)(DG)(DT)(DG)(DA)(DC)(DA)

GTGGGGCCACTAGGGACAGGATTGGTGACA
DNA #4 30 bases

(DT)(DG)(DT)(DC)(DA)(DC)(DC)(DA)(DA)(DT)(DC)(DC)(DT)(DG)(DT)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DG)(DG)(DC)(DC)(DC)(DC)(DA)(DC)

TGTCACCAATCCTGTCCCTAGTGGCCCCAC
Non polymer #5 MG

InChI=1S/Mg/q+2

[Mg+2]
Download coordinates
Reference