2025-06-28_00000187|hard|9KLN

Cryo-EM structure of ChCas12b-sgRNA-target DNA ternary complex (Complex-A); Electron microscopy 2.55Å
Protein #1 1496 residues (93% resolved)

PADDLSTQRAYTLRLQGTDPEDQSWRDALWMTHEAVNAGGRAFGDWLLTLRGGIAHELADTPVKGKKDITDELRKKRRILLALSWLSVESRRGAPDKFIVAGGEEPAGSRNEKVLQALKEILKRRGLSAEESESWMSDCRASLSAAIRDDAVWVNRSAAFDDAQVRIGASLTREDIWDMLDPFFGSREAYLTPAKKKKEDEDSSEGTGEEKAKDLVQKAGQWLSSRFGTGKGANFDAMAEVYSKISEWAGTAQEGVSGKEGIKNLADALAAFSPVSQNLEGVLKLISGPGYKSATRNLLGELDSLPVVSRDHLSALHEKAAEDTVKCKESTGTKGRRPYADAILNDVEKRCGFTYLTDSDNRSVSILDTSEFPSDYKWGTARHSEFAVILDHAARRISVAHSWIKLAEAERDRCEEDAAKVYDLPDKVKEWLDTFCSNRSDISGAQGEGYRIRRKAIEGWKEVVASWGRSSCITAEDRVAAARALQDDPEIDKFGAIQLFEILAQDEALCVWHKDGDVAKSPDAQMLIDYVLASDAESKKRRFKVPAYRHPDALLHPIFCDFGNSRWDITYDIHGARGKKKAKRGSKKEEAMPRGVAMKLWTGSDVLSVSLRWQSKKLAADLALDQEAEEVTDTAAVSRADRLGRAAAGIDRGAGVTIAGLFEEAHWNGRLQAPRQQLEAIAAVRDNQKLSSEERERRIAFMKDRIRWLVTFSAKLRPQGPWHSYAPTQGLQSDPKYWPHSEINKKRKGQAKLILSRLPGLRILSVDLGHRFAAACAVWETMSSEAIQEACRLANHQLPAPADLYLHLKRTVQKNLIDGEKTVEESTVYRRIGADRLPDGTAHPAPWARLDRQFLIKLQGEEKVREASNEEVWQVHLMESALGLSFPLIDRLVYAGWGGTEKQAARLEALREKGWKPTGTPADQDEEGGGYKPSLAVDELMFSAVRTLRLALKYHGDRARIAFALTADYKPMPGDTRYYFSEAKDRSSGADAAEREAKHKDYLLDMLLLWHDLAFSRKWRDEEAKELWNLHIAALPGYQAPAAPIQEEAGQGRKKAREEARAKMTPAAEALLADGTLREKLHGLWKERWEKDDAQWKKHLRWMKDGILPRGGRAATPSIRYVGGLSLTRLATLTEFRRKVQVGFYTRLFPSGEKREIKEAFGQTALDALERLREQRVKQLASRIAEAALGAGRVSRTALKQDPKRPEARVDAACHAVIIENLEHYRPEETRTRRENRGLMNWASSKVKKYLSEACQLHGLFLREVPAGYTSRQDSRTGAPGMRCQDVTVKTFLNSPFWQKQCVQAQKNKSTARDRFLCALKEAVAQGGMEEEKKMGPIRVPVPGGEVFVSADAASPAAKGLQADLNAAANIGLRALLDPDWPGKWWYVPCDRKTAYPAKEKVEGSAAVDVKQALPFVLPEEKENKGKTKGGKKGKGEVMNLWRDVSAEPLMTGQWLDYTAYRKEVENRVIQVLTAQLKARNPLRFGNLGDEEEIPY

PADDLSTQRAYTLRLQGTDPEDQSWRDALWMTHEAVNAGGRAFGDWLLTLRGGIAHELADTPVKGKKDITDELRKKRRILLALSWLSVESRRGAPDKFIVAGGEEPAGSRNEKVLQALKEILKRRGLSAEESESWMSDCRASLSAAIRDDAVWVNRSAAFDDAQVRIGASLTREDIWDMLDPFFGSREAYLTPAKKKKEDEDSSEGTGEEKAKDLVQKAGQWLSSRFGTGKGANFDAMAEVYSKISEWAGTAQEGVSGKEGIKNLADALAAFSPVSQNLEGVLKLISGPGYKSATRNLLGELDSLPVVSRDHLSALHEKAAEDTVKCKESTGTKGRRPYADAILNDVEKRCGFTYLTDSDNRSVSILDTSEFPSDYKWGTARHSEFAVILDHAARRISVAHSWIKLAEAERDRCEEDAAKVYDLPDKVKEWLDTFCSNRSDISGAQGEGYRIRRKAIEGWKEVVASWGRSSCITAEDRVAAARALQDDPEIDKFGAIQLFEILAQDEALCVWHKDGDVAKSPDAQMLIDYVLASDAESKKRRFKVPAYRHPDALLHPIFCDFGNSRWDITYDIHGARGKKKAKRGSKKEEAMPRGVAMKLWTGSDVLSVSLRWQSKKLAADLALDQEAEEVTDTAAVSRADRLGRAAAGIDRGAGVTIAGLFEEAHWNGRLQAPRQQLEAIAAVRDNQKLSSEERERRIAFMKDRIRWLVTFSAKLRPQGPWHSYAPTQGLQSDPKYWPHSEINKKRKGQAKLILSRLPGLRILSVDLGHRFAAACAVWETMSSEAIQEACRLANHQLPAPADLYLHLKRTVQKNLIDGEKTVEESTVYRRIGADRLPDGTAHPAPWARLDRQFLIKLQGEEKVREASNEEVWQVHLMESALGLSFPLIDRLVYAGWGGTEKQAARLEALREKGWKPTGTPADQDEEGGGYKPSLAVDELMFSAVRTLRLALKYHGDRARIAFALTADYKPMPGDTRYYFSEAKDRSSGADAAEREAKHKDYLLDMLLLWHDLAFSRKWRDEEAKELWNLHIAALPGYQAPAAPIQEEAGQGRKKAREEARAKMTPAAEALLADGTLREKLHGLWKERWEKDDAQWKKHLRWMKDGILPRGGRAATPSIRYVGGLSLTRLATLTEFRRKVQVGFYTRLFPSGEKREIKEAFGQTALDALERLREQRVKQLASRIAEAALGAGRVSRTALKQDPKRPEARVDAACHAVIIENLEHYRPEETRTRRENRGLMNWASSKVKKYLSEACQLHGLFLREVPAGYTSRQDSRTGAPGMRCQDVTVKTFLNSPFWQKQCVQAQKNKSTARDRFLCALKEAVAQGGMEEEKKMGPIRVPVPGGEVFVSADAASPAAKGLQADLNAAANIGLRALLDPDWPGKWWYVPCDRKTAYPAKEKVEGSAAVDVKQALPFVLPEEKENKGKTKGGKKGKGEVMNLWRDVSAEPLMTGQWLDYTAYRKEVENRVIQVLTAQLKARNPLRFGNLGDEEEIPY
RNA #2 115 bases

GCUUCUACAGGAGGCGAAAAGACUGCGGAACGUGUCUUCCCCUUCAAUGGGCGUGGCACCGCAGCGUUGUUCAGUCCUGAUCAACGGACACGGAUAUGUUGAAGAACACCAUGAC

GCUUCUACAGGAGGCGAAAAGACUGCGGAACGUGUCUUCCCCUUCAAUGGGCGUGGCACCGCAGCGUUGUUCAGUCCUGAUCAACGGACACGGAUAUGUUGAAGAACACCAUGAC
DNA #3 33 bases

(DG)(DT)(DC)(DA)(DT)(DG)(DG)(DT)(DG)(DT)(DT)(DC)(DT)(DT)(DC)(DA)(DA)(DC)(DA)(DT)(DA)(DT)(DC)(DC)(DC)(DA)(DA)(DC)(DG)(DC)(DA)(DT)(DG)

GTCATGGTGTTCTTCAACATATCCCAACGCATG
DNA #4 9 bases

(DC)(DA)(DT)(DG)(DC)(DG)(DT)(DT)(DG)

CATGCGTTG
Non polymer #5 MG

InChI=1S/Mg/q+2

[Mg+2]
Download coordinates
Reference