2025-04-19_00000361|hard|9N69

Structure of the retron IA complex with HNH nuclease in the \"down\" orientation; Electron microscopy 3.13Å
Protein #1 552 residues (95% resolved)

MWSHPQFEKINKMNLETCYVDFLELESHVINEDYLKESVELQKLISTLNESKFHLNKIGIHDFKRIRELQISLEDDLTVFVGDNGFGKSTILDAIAIVLSWLRSNIEKESKPGTYIKSHEVNNSVDVEYASIDANIKLKDFNTSILITKAKEGAYYSRNNELLGVKKLASIYRLVNKYVDNASLPLMAYYSIARSYIGGGVDRKRKNAKTKTVWSKFDVYDEIEFDRNDFTDFFQWLVFLHNRASQEKLSESQTTINALFSDIQSLKATLTQLSAIDNIDSTVIKGLELSLKEKLNYMKSLQSGEHKFNNAVSLYDSVINTILKFLPEFQWIKLVYGDDDYKIILKKGEVELDIQQLSQGEKTIFTLVGDLARRLILLNPNLSNPLLGYGIVLIDEIDLHLHPQWQQTIIERLTSTFPNVQFVITTHSPQVLSTVSSRSVRILQEVEVDGVNDLIVSHPDYQIKGVSNQDALLYGMRTDPIPSTKENGWLEEYKKLVELNRYSSDEALLLREKVIKHFGLDHPLVQECDDLISVLEFKNKINQHFSGSKDVK

MWSHPQFEKINKMNLETCYVDFLELESHVINEDYLKESVELQKLISTLNESKFHLNKIGIHDFKRIRELQISLEDDLTVFVGDNGFGKSTILDAIAIVLSWLRSNIEKESKPGTYIKSHEVNNSVDVEYASIDANIKLKDFNTSILITKAKEGAYYSRNNELLGVKKLASIYRLVNKYVDNASLPLMAYYSIARSYIGGGVDRKRKNAKTKTVWSKFDVYDEIEFDRNDFTDFFQWLVFLHNRASQEKLSESQTTINALFSDIQSLKATLTQLSAIDNIDSTVIKGLELSLKEKLNYMKSLQSGEHKFNNAVSLYDSVINTILKFLPEFQWIKLVYGDDDYKIILKKGEVELDIQQLSQGEKTIFTLVGDLARRLILLNPNLSNPLLGYGIVLIDEIDLHLHPQWQQTIIERLTSTFPNVQFVITTHSPQVLSTVSSRSVRILQEVEVDGVNDLIVSHPDYQIKGVSNQDALLYGMRTDPIPSTKENGWLEEYKKLVELNRYSSDEALLLREKVIKHFGLDHPLVQECDDLISVLEFKNKINQHFSGSKDVK
Protein #2 311 residues (99% resolved)

MQLTSKIISKFNYNRLAFQLLLNEAPKKYKVYYIPKRGAGFRVIAQPTKELKNVQRFIVSLLQPKLPVHHKAMAYEYKKSIKDNALLHKDNNYILKMDFQNFFNKIKPDIFFSKLENTGLKLDSFDENTLRNLLFWRPGKKRSTTLILSVGAPSSPFISNFVMYDFDKSLDDWCRNNGITYSRYADDITFSTNIKDILCRVPKVVKKMLSLHVPGLSINESKTIFTSMAHNRHVTGVTLTPQGNLSIGRDRKRMLFAKIHKYSLGLLSSEEINKTKGMIAFANYLEGDFLLRLQKKYGCELITKFLMEGNK

MQLTSKIISKFNYNRLAFQLLLNEAPKKYKVYYIPKRGAGFRVIAQPTKELKNVQRFIVSLLQPKLPVHHKAMAYEYKKSIKDNALLHKDNNYILKMDFQNFFNKIKPDIFFSKLENTGLKLDSFDENTLRNLLFWRPGKKRSTTLILSVGAPSSPFISNFVMYDFDKSLDDWCRNNGITYSRYADDITFSTNIKDILCRVPKVVKKMLSLHVPGLSINESKTIFTSMAHNRHVTGVTLTPQGNLSIGRDRKRMLFAKIHKYSLGLLSSEEINKTKGMIAFANYLEGDFLLRLQKKYGCELITKFLMEGNK
Protein #3 227 residues (99% resolved)

MKYLSRQMPGPSVLNKFDYRRDDWNSLSSNDKKEIWEEIIKMQGKLCAYCEKKIEHHKSGGKNKVERHIEHFYRKSYYKNLTFEWSNLFGSCGEPQRCGFYKDKQKYNDDDLIKADRQNPDVFFHFLENGDVHIREGLNEKEHKMAEVTLRVFNLNPSSGGVKAERRRAIELSMTLIKELVGCASQLIESGCEIEDVRSMVFDEFKKNVKDRCFTTAIKHVFENRMP

MKYLSRQMPGPSVLNKFDYRRDDWNSLSSNDKKEIWEEIIKMQGKLCAYCEKKIEHHKSGGKNKVERHIEHFYRKSYYKNLTFEWSNLFGSCGEPQRCGFYKDKQKYNDDDLIKADRQNPDVFFHFLENGDVHIREGLNEKEHKMAEVTLRVFNLNPSSGGVKAERRRAIELSMTLIKELVGCASQLIESGCEIEDVRSMVFDEFKKNVKDRCFTTAIKHVFENRMP
DNA #4 162 bases

(DC)(DT)(DC)(DT)(DT)(DC)(DA)(DC)(DT)(DA)(DA)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DA)(DA)(DA)(DG)(DA)(DC)(DA)(DC)(DA)(DG)(DA)(DT)(DT)(DT)(DC)(DT)(DC)(DC)(DT)(DT)(DC)(DG)(DC)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DC)(DC)(DC)(DC)(DG)(DG)(DG)(DC)(DA)(DG)(DG)(DG)(DA)(DT)(DG)(DC)(DG)(DA)(DA)(DG)(DG)(DA)(DG)(DA)(DA)(DA)(DT)(DC)(DT)(DG)(DT)(DG)(DT)(DC)(DT)(DT)(DT)(DC)(DG)(DC)(DA)(DA)(DC)(DC)(DC)(DT)(DA)(DA)(DA)(DC)(DC)(DG)(DG)(DA)(DC)(DA)(DA)(DT)(DA)(DA)(DA)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DC)(DT)(DG)(DG)(DC)(DT)(DA)(DA)(DT)(DC)(DA)(DC)(DC)(DC)(DG)(DG)(DC)(DT)(DG)(DG)(DA)(DA)(DC)(DC)(DA)(DA)(DT)(DG)(DT)(DT)(DC)(DC)(DT)(DA)(DC)(DA)(DC)(DT)(DA)(DA)(DA)(DG)(DA)(DG)(DC)

CTCTTCACTAAAGACAGCGAAAGACACAGATTTCTCCTTCGCATATCTGCCCCGGGCAGGGATGCGAAGGAGAAATCTGTGTCTTTCGCAACCCTAAACCGGACAATAAATTTAAGCCTGGCTAATCACCCGGCTGGAACCAATGTTCCTACACTAAAGAGC
RNA #5 162 bases

GCUCUUUAGUGUAGGAAUAUUGGUUCCAGCCGGGUGAUUAGCCAGGCUUAAAUUUAUUGUCCGGUUUAGGGUUGCGAAAGACACAGAUUUCUCCUUCGCAUCCCUGCCCGGGGCAGAUAUGCGAAGGAGAAAUCUGUGUCUUUCGCUGUCUUUAGUGAAGAG

GCUCUUUAGUGUAGGAAUAUUGGUUCCAGCCGGGUGAUUAGCCAGGCUUAAAUUUAUUGUCCGGUUUAGGGUUGCGAAAGACACAGAUUUCUCCUUCGCAUCCCUGCCCGGGGCAGAUAUGCGAAGGAGAAAUCUGUGUCUUUCGCUGUCUUUAGUGAAGAG
Non polymer #6 ATP

InChI=1S/C10H16N5O13P3/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1

c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@@](=O)(O)O[P@](=O)(O)OP(=O)(O)O)O)O)N
Non polymer #7 ZN

InChI=1S/Zn/q+2

[Zn+2]
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Reference