2025-04-12_00000040|hard|8WRS

Cryo-EM structure of Cas12-1 with 5 nt complementary heteroduplex; Electron microscopy 2.93Å
Protein #1 737 residues (83% resolved)

MTPKTETPVGALIKKFFPGKRFQKNYLKDAGKKLKREGEAAAVEYLSGKQEDHPANFCPPAKVNILAQSRPLSEWPINLVSKGVQEYVYGLTAAEREANGDFGTSRKSLDRWFARTGVPTHGYTTVQGLNLILRHTFNRYDGVIKKVETRNEKRRSKATRINVSREADGLPPIEAEPEETAFGPDGKLKERPGINPSIYCYQQVSPVPYNPAKHPALPFSGVDPGAPLPLGTPNRLSIPKGQPGYVPEWQRPHLSTKNKRIRKWYARANWRRKPGRKSVLDEAKLKEAALKEAIPIIVTIGKDWIVMDARGLLRAVYWRGIAKPGLSLKELLGFFSGDPVLDPKRGIATFTFKLGAVAVHSRKPTRGKKSKELLLSMTAEKPHVGLVAIDLGQTNPVAAEFSRVKREGETLQAEPLGQIVLPDDLVKDLTRYRRAWDATEEQIKAEAIVQLPEECRAEVVKVNQMSAEETKHLILDRGVSGDLPWEKMTSNTTFISDHLLAKGVTDQVFFEKKSKGKKKGTETVKRKDYGWVKLLRPRLSQETRKAVNDKTWELKRASTEYVRLSRRKTELARRCVNYIVRETKRWTQCEDIAIVIEDLNVRFFHGSGERPDGWDNFFISKRENRWFIQVLHKAFSDLALHRGLPVIEANPARTSITCIRCGHCDRNNRHGEMFLCLSCNDLRHADREIATRNLTRVAVTGEMIPRRIEPGEQSGDTKKARSARKGKKAVISKREAA

MTPKTETPVGALIKKFFPGKRFQKNYLKDAGKKLKREGEAAAVEYLSGKQEDHPANFCPPAKVNILAQSRPLSEWPINLVSKGVQEYVYGLTAAEREANGDFGTSRKSLDRWFARTGVPTHGYTTVQGLNLILRHTFNRYDGVIKKVETRNEKRRSKATRINVSREADGLPPIEAEPEETAFGPDGKLKERPGINPSIYCYQQVSPVPYNPAKHPALPFSGVDPGAPLPLGTPNRLSIPKGQPGYVPEWQRPHLSTKNKRIRKWYARANWRRKPGRKSVLDEAKLKEAALKEAIPIIVTIGKDWIVMDARGLLRAVYWRGIAKPGLSLKELLGFFSGDPVLDPKRGIATFTFKLGAVAVHSRKPTRGKKSKELLLSMTAEKPHVGLVAIDLGQTNPVAAEFSRVKREGETLQAEPLGQIVLPDDLVKDLTRYRRAWDATEEQIKAEAIVQLPEECRAEVVKVNQMSAEETKHLILDRGVSGDLPWEKMTSNTTFISDHLLAKGVTDQVFFEKKSKGKKKGTETVKRKDYGWVKLLRPRLSQETRKAVNDKTWELKRASTEYVRLSRRKTELARRCVNYIVRETKRWTQCEDIAIVIEDLNVRFFHGSGERPDGWDNFFISKRENRWFIQVLHKAFSDLALHRGLPVIEANPARTSITCIRCGHCDRNNRHGEMFLCLSCNDLRHADREIATRNLTRVAVTGEMIPRRIEPGEQSGDTKKARSARKGKKAVISKREAA
RNA #2 46 bases

UCAACGCUUGCUCGGUUCGCCGAGACUCCCCUACGUGCUGCUGAAG

UCAACGCUUGCUCGGUUCGCCGAGACUCCCCUACGUGCUGCUGAAG
DNA #3 42 bases

(DC)(DT)(DG)(DC)(DC)(DC)(DT)(DT)(DG)(DC)(DA)(DA)(DG)(DA)(DA)(DG)(DT)(DC)(DG)(DT)(DC)(DG)(DT)(DG)(DC)(DA)(DT)(DG)(DG)(DG)(DG)(DA)(DG)(DA)(DA)(DT)(DT)(DG)(DG)(DC)(DC)(DA)

CTGCCCTTGCAAGAAGTCGTCGTGCATGGGGAGAATTGGCCA
DNA #4 42 bases

(DT)(DG)(DG)(DC)(DC)(DA)(DA)(DT)(DT)(DC)(DT)(DC)(DC)(DC)(DC)(DA)(DT)(DG)(DC)(DA)(DC)(DG)(DA)(DC)(DG)(DA)(DC)(DT)(DT)(DC)(DT)(DT)(DG)(DC)(DA)(DA)(DG)(DG)(DG)(DC)(DA)(DG)

TGGCCAATTCTCCCCATGCACGACGACTTCTTGCAAGGGCAG
Download coordinates
Reference