2025-03-15_00000233|hard|9C51

Cryo-EM structure of the Strand displacement Complex (IV) of Yeast Mitochondrial DNA polymerase Gamma (MIP1) with downstream DNA; Electron microscopy 2.42Å
Protein #1 1240 residues (81% resolved)

MASTKKNTAEAPRINPVGIQYLGESLQRQVFGSCGGKDEVEQSDKLMELSKKSLKDHGLWGKKTLITDPISFPLPPLQGRSLDEHFQKIGRFNSEPYKSFCEDKFTEMVARPAEWLRKPGWVKYVPGMAPVEVAYPDEELVVFDVETLYNVSDYPTLATALSSTAWYLWCSPFICGGDDPAALIPLNTLNKEQVVIGHNVAYDRARVLEEYNFRDSKAFFLDTQSLHIASFGLCSRQRPMFMKNNKKKEAEVESEVHPEISIEDYDDPWLNVSALNSLKDVAKFHCKIDLDKTDRDFFASTDKSTIIENFQKLVNYCATDVTATSQVFDKIFPVFLKKCPHPVSFAGLKSLSKCILPTKLNDWNDYLNSSESLYQQSKVQIESKIVQIIKDIALLKDKPDFYLKDPWLSQLDWTTKPLRLTKKGVPAKCQKLPGFPEWYRQLFPSKDTVEPKITIKSRIIPILFKLSWENSPVIWSKESGWCFNVPHEQVETYKAKNYVLADSVSQEEEEIRMNNLGLQCTGVLFKVPHPNGPTFNCTNLLTKSYNHFFEKGVLKSESELAHQALQINSSGSYWMSARERIQSQFVVPNCKFPNEFQSLSAKSSLNNEKTNDLAIIIPKIVPMGTITRRAVENTWLTASNAKANRIGSELKTQVKAPPGYCFVGADVDSEELWIASLVGDSIFNVHGGTAIGWMCLEGTKNEGTDLHTKTAQILGCSRNEAKIFNYGRIYGAGAKFASQLLKRFNPSLTDEETKKIANKLYENTKGKTKRSKLFKKFWYGGSESILFNKLESIAEQETPKTPVLGCGITYSLMKKNLRANSFLPSRINWAIQSSGVDYLHLLCCSMEYIIKKYNLEARLCISIHDEIRFLVSEKDKYRAAMALQISNIWTRAMFCQQMGINELPQNCAFFSQVDIDSVIRKEVNMDCITPSNKTAIPHGEALDINQLLDKPNSKLGKPSLDIDSKVSQYAYNYREPVFEEYNKSYTPEFLKYFLAMQVQSDKRDVNRLEDEYLRECTSKEYARDGNTAEYSLLDYIKDVEKGKRTKVRIMGSNFLDGTKNAKADQRIRLPVNMPDYPTLHKIANDSAIPEKQLLENRRKKENRIDDENKKKLTRKKNTTPMERKYKRVYGGRKAFEAFYECANKPLDYTLETEKQFFNIPIDGVIDDVLNDKSNYKKKPSQARTASSSPIRKTAKAVHSKKLPARKSSTTNRNLVELERDITISREYKLAAALEHHHHHH

MASTKKNTAEAPRINPVGIQYLGESLQRQVFGSCGGKDEVEQSDKLMELSKKSLKDHGLWGKKTLITDPISFPLPPLQGRSLDEHFQKIGRFNSEPYKSFCEDKFTEMVARPAEWLRKPGWVKYVPGMAPVEVAYPDEELVVFDVETLYNVSDYPTLATALSSTAWYLWCSPFICGGDDPAALIPLNTLNKEQVVIGHNVAYDRARVLEEYNFRDSKAFFLDTQSLHIASFGLCSRQRPMFMKNNKKKEAEVESEVHPEISIEDYDDPWLNVSALNSLKDVAKFHCKIDLDKTDRDFFASTDKSTIIENFQKLVNYCATDVTATSQVFDKIFPVFLKKCPHPVSFAGLKSLSKCILPTKLNDWNDYLNSSESLYQQSKVQIESKIVQIIKDIALLKDKPDFYLKDPWLSQLDWTTKPLRLTKKGVPAKCQKLPGFPEWYRQLFPSKDTVEPKITIKSRIIPILFKLSWENSPVIWSKESGWCFNVPHEQVETYKAKNYVLADSVSQEEEEIRMNNLGLQCTGVLFKVPHPNGPTFNCTNLLTKSYNHFFEKGVLKSESELAHQALQINSSGSYWMSARERIQSQFVVPNCKFPNEFQSLSAKSSLNNEKTNDLAIIIPKIVPMGTITRRAVENTWLTASNAKANRIGSELKTQVKAPPGYCFVGADVDSEELWIASLVGDSIFNVHGGTAIGWMCLEGTKNEGTDLHTKTAQILGCSRNEAKIFNYGRIYGAGAKFASQLLKRFNPSLTDEETKKIANKLYENTKGKTKRSKLFKKFWYGGSESILFNKLESIAEQETPKTPVLGCGITYSLMKKNLRANSFLPSRINWAIQSSGVDYLHLLCCSMEYIIKKYNLEARLCISIHDEIRFLVSEKDKYRAAMALQISNIWTRAMFCQQMGINELPQNCAFFSQVDIDSVIRKEVNMDCITPSNKTAIPHGEALDINQLLDKPNSKLGKPSLDIDSKVSQYAYNYREPVFEEYNKSYTPEFLKYFLAMQVQSDKRDVNRLEDEYLRECTSKEYARDGNTAEYSLLDYIKDVEKGKRTKVRIMGSNFLDGTKNAKADQRIRLPVNMPDYPTLHKIANDSAIPEKQLLENRRKKENRIDDENKKKLTRKKNTTPMERKYKRVYGGRKAFEAFYECANKPLDYTLETEKQFFNIPIDGVIDDVLNDKSNYKKKPSQARTASSSPIRKTAKAVHSKKLPARKSSTTNRNLVELERDITISREYKLAAALEHHHHHH
DNA #2 23 bases

(DT)(DT)(DT)(DT)(DG)(DG)(DT)(DA)(DG)(DT)(DC)(DG)(DT)(DG)(DA)(DC)(DT)(DC)(DG)(DA)(DC)(DC)(DG)

TTTTGGTAGTCGTGACTCGACCG
DNA #3 24 bases

(DG)(DA)(DA)(DG)(DA)(DC)(DA)(DG)(DT)(DC)(DT)(DG)(DC)(DG)(DG)(DC)(DG)(DC)(DG)(DC)(DG)(DG)(DG)(2DT)

GAAGACAGTCTGCGGCGCGCGGGT
DNA #4 42 bases

(DC)(DG)(DG)(DT)(DC)(DG)(DA)(DG)(DA)(DG)(DT)(DC)(DA)(DC)(DG)(DA)(DC)(DT)(DA)(DC)(DC)(DC)(DG)(DC)(DG)(DC)(DG)(DC)(DC)(DG)(DC)(DA)(DG)(DA)(DC)(DT)(DG)(DT)(DC)(DT)(DT)(DC)

CGGTCGAGAGTCACGACTACCCGCGCGCCGCAGACTGTCTTC
Non polymer #5 DTP

InChI=1S/C10H16N5O12P3/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19)/t5-,6+,7+/m0/s1

c1nc(c2c(n1)n(cn2)[C@H]3C[C@@H]([C@H](O3)CO[P@](=O)(O)O[P@@](=O)(O)OP(=O)(O)O)O)N
Non polymer #6 MG

InChI=1S/Mg/q+2

[Mg+2]
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Reference