2024-09-28_00000074|hard|8UMF

Structure of PsCas9 in complex with gRNA and DNA in product state; Electron microscopy 2.90Å
Protein #1 1462 residues (94% resolved)

GAASMKRTADGSEFESPKKKRKVTVKSTLAVDMGGRYTGIFSYTTDSGFPKAKEARAYVLNMPDNDALTYSMAARTQTRHRIRSQQRFVLARRLTYILIEGKLKRKLSPREKEAISSLLRRRGYSRLESELDLSVLQGVESGFFKCFLPNFDEDENLLTQWTSLTDGYLQNNSDSRRQIQIFLESSKDSKEFLTVVKSQHQDTKEYKNALKVMRDDAESMIEQSMFGHKHRRLYLEAIAQDIPRDSRLKPIIEAFSGVEKFHHFIGNLSNLQLRALRWYFNDPSMKNNVFDKERLKSVLVRAYQFFHYPKDLTQQRAEVLNAYEGATDILETLQTLNPELTIPPYEDQNNRRPPLDQTLWLSPRLLDQRYGDTWEIWVQNLLRSPLSKGIDENLDTILITTDRKARLLERQSGRLIHYTSQKLYHSYVLQRLLDRTVENDAYLLKTLVSSNRGNSNEIHQAQERLTRDLGSQHIKKFLDFVRQYYDEVDKAKRGLWFIVEKPLMERADIHPPMKNDSVILRLVGNILCVSDLVDLSFWTRKVKGQSTVRSLCTAIEKTRKEYGNSFNYLYQRALYLQSKGKKLSAEDKDFIKLQSNVLLVSDVIAEALDIKEEQKKKFANPFSLAQLYNIIETEKSGFISTTLAAVDENAWRNNLQGKARCVQLCADTVRPFDGALRNILDRQAYEIAKLKAEELLSTELKNQTIDLVVLLESNQFAFSASLAEVKKSANTAAIRQKVAKAQKRQQDRWLSKDERIKSASRGLCPYTGKNLGDKGEVDHIIPRSLSMNYMGSILNSEANLIYCSQEGNQLKLNGRKKLSDLADNYLKVVFGTADRGTICKYIEKSVSELTDAKIVQFELLDRSQQDAVRHALFLEDFSEARRRIIRLLGKINTARVNGTQAWFAKSFITKLRELTKEWCANNQITLAFDLYRLDAQTVSQDYRKKFALINKDWAKPDDKKQPIASHAIDAFCVFAAAKDKRNIANVLGVFDEVAEEQNLKTIAQLMPSEVNLISPKRKSILDKNEVGSRALMKEGIFAEHFLPILVRGDDCRIGFDWSESGSVKVKDADKLFGVLDGLLKQSQKRSVNGFETYTVDRIKAFELLHDVFIRPCSQKMLEQAEVLEKLHYITQNISVTSVYDAVNRQFKCREEILKDKDFDIKVDLGNRFGSAKGKITLPAKREWEKLVNRSELKNLIKDKLSDKGSEKTPDGETLIYDIFRSIPVQKLSHKATRRVWSLPKIPSISSGVRIKRKDSNGNDIYQLYMLNDTKCKGFVVNEKGVIDWSSDLVADLYKQPTLTILNGRYLKADQYVRMDQWYEVDCGRDDVIVKMCPGTSGRRYIEITQSKKQFEDWTGYISGSFWNYPVTIKLSSQQIANFVKNSQMPLLGKPRSGQITVITLGNTLKYWYCVESKNSMMNEAYQKAYLVHFNQSGGSASDYKDDDDKKRTADGSEFEPKKKRKV

GAASMKRTADGSEFESPKKKRKVTVKSTLAVDMGGRYTGIFSYTTDSGFPKAKEARAYVLNMPDNDALTYSMAARTQTRHRIRSQQRFVLARRLTYILIEGKLKRKLSPREKEAISSLLRRRGYSRLESELDLSVLQGVESGFFKCFLPNFDEDENLLTQWTSLTDGYLQNNSDSRRQIQIFLESSKDSKEFLTVVKSQHQDTKEYKNALKVMRDDAESMIEQSMFGHKHRRLYLEAIAQDIPRDSRLKPIIEAFSGVEKFHHFIGNLSNLQLRALRWYFNDPSMKNNVFDKERLKSVLVRAYQFFHYPKDLTQQRAEVLNAYEGATDILETLQTLNPELTIPPYEDQNNRRPPLDQTLWLSPRLLDQRYGDTWEIWVQNLLRSPLSKGIDENLDTILITTDRKARLLERQSGRLIHYTSQKLYHSYVLQRLLDRTVENDAYLLKTLVSSNRGNSNEIHQAQERLTRDLGSQHIKKFLDFVRQYYDEVDKAKRGLWFIVEKPLMERADIHPPMKNDSVILRLVGNILCVSDLVDLSFWTRKVKGQSTVRSLCTAIEKTRKEYGNSFNYLYQRALYLQSKGKKLSAEDKDFIKLQSNVLLVSDVIAEALDIKEEQKKKFANPFSLAQLYNIIETEKSGFISTTLAAVDENAWRNNLQGKARCVQLCADTVRPFDGALRNILDRQAYEIAKLKAEELLSTELKNQTIDLVVLLESNQFAFSASLAEVKKSANTAAIRQKVAKAQKRQQDRWLSKDERIKSASRGLCPYTGKNLGDKGEVDHIIPRSLSMNYMGSILNSEANLIYCSQEGNQLKLNGRKKLSDLADNYLKVVFGTADRGTICKYIEKSVSELTDAKIVQFELLDRSQQDAVRHALFLEDFSEARRRIIRLLGKINTARVNGTQAWFAKSFITKLRELTKEWCANNQITLAFDLYRLDAQTVSQDYRKKFALINKDWAKPDDKKQPIASHAIDAFCVFAAAKDKRNIANVLGVFDEVAEEQNLKTIAQLMPSEVNLISPKRKSILDKNEVGSRALMKEGIFAEHFLPILVRGDDCRIGFDWSESGSVKVKDADKLFGVLDGLLKQSQKRSVNGFETYTVDRIKAFELLHDVFIRPCSQKMLEQAEVLEKLHYITQNISVTSVYDAVNRQFKCREEILKDKDFDIKVDLGNRFGSAKGKITLPAKREWEKLVNRSELKNLIKDKLSDKGSEKTPDGETLIYDIFRSIPVQKLSHKATRRVWSLPKIPSISSGVRIKRKDSNGNDIYQLYMLNDTKCKGFVVNEKGVIDWSSDLVADLYKQPTLTILNGRYLKADQYVRMDQWYEVDCGRDDVIVKMCPGTSGRRYIEITQSKKQFEDWTGYISGSFWNYPVTIKLSSQQIANFVKNSQMPLLGKPRSGQITVITLGNTLKYWYCVESKNSMMNEAYQKAYLVHFNQSGGSASDYKDDDDKKRTADGSEFEPKKKRKV
RNA #2 131 bases

AUGUCACCUCCAAUGACUAGGGGUUUCAGUUAUUCGUGAAAACGAAUGAAGUCACUCUUAAAGUGAGCUGAAAUCACUAAAAAUUAAGAUUGAACCCGGCUACUGACUCUGUCAUCCGGGUUUACUUAUUU

AUGUCACCUCCAAUGACUAGGGGUUUCAGUUAUUCGUGAAAACGAAUGAAGUCACUCUUAAAGUGAGCUGAAAUCACUAAAAAUUAAGAUUGAACCCGGCUACUGACUCUGUCAUCCGGGUUUACUUAUUU
DNA #3 80 bases

(DT)(DA)(DA)(DG)(DC)(DA)(DG)(DG)(DC)(DC)(DA)(DA)(DT)(DG)(DG)(DG)(DG)(DA)(DG)(DG)(DA)(DC)(DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DC)(DA)(DC)(DC)(DT)(DC)(DC)(DA)(DA)(DT)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DT)(DG)(DG)(DG)(DC)(DA)(DA)(DC)(DC)(DA)(DC)(DA)(DA)(DA)(DC)(DC)(DC)(DA)(DC)(DG)(DA)(DG)(DG)(DG)(DC)(DA)(DG)(DA)(DG)(DT)(DG)(DC)

TAAGCAGGCCAATGGGGAGGACATCGATGTCACCTCCAATGACTAGGGTGGGCAACCACAAACCCACGAGGGCAGAGTGC
DNA #4 80 bases

(DG)(DC)(DA)(DC)(DT)(DC)(DT)(DG)(DC)(DC)(DC)(DT)(DC)(DG)(DT)(DG)(DG)(DG)(DT)(DT)(DT)(DG)(DT)(DG)(DG)(DT)(DT)(DG)(DC)(DC)(DC)(DA)(DC)(DC)(DC)(DT)(DA)(DG)(DT)(DC)(DA)(DT)(DT)(DG)(DG)(DA)(DG)(DG)(DT)(DG)(DA)(DC)(DA)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DC)(DC)(DT)(DC)(DC)(DC)(DC)(DA)(DT)(DT)(DG)(DG)(DC)(DC)(DT)(DG)(DC)(DT)(DT)(DA)

GCACTCTGCCCTCGTGGGTTTGTGGTTGCCCACCCTAGTCATTGGAGGTGACATCGATGTCCTCCCCATTGGCCTGCTTA
Non polymer #5 MG

InChI=1S/Mg/q+2

[Mg+2]
Download coordinates
Reference