2024-02-24_00000209|hard|8W9M

Cryo-EM structure of the cyanobacterial nitrate transporter NrtBCD in complex with ATP; Electron microscopy 3.10Å
Protein #1 279 residues (88% resolved)

MTAVLGNRARVRKSQKAINNFLWKKVVPPLVALGIFLVIWQLLCLNPNFKLPGPIETFSETWDPFIINPFFDNGESDKGLGWQILSSLGRVGLGFSLAAIAGIILGILIGVNPLVYNAVDPIFQVLRTVPPLAWLPISLAAFQQANPSAIFVIFITSIWPILLNTTVGVQQIPQDYINVAKVLRLKGVKYFFKIVFPATVPYIFTGLRIGIGLSWLAIVAAEMLVGGVGIGSFIWDAYNTTTETNLSEIILALIYVGLVGLLLDRLVGFVASKVVADQK

MTAVLGNRARVRKSQKAINNFLWKKVVPPLVALGIFLVIWQLLCLNPNFKLPGPIETFSETWDPFIINPFFDNGESDKGLGWQILSSLGRVGLGFSLAAIAGIILGILIGVNPLVYNAVDPIFQVLRTVPPLAWLPISLAAFQQANPSAIFVIFITSIWPILLNTTVGVQQIPQDYINVAKVLRLKGVKYFFKIVFPATVPYIFTGLRIGIGLSWLAIVAAEMLVGGVGIGSFIWDAYNTTTETNLSEIILALIYVGLVGLLLDRLVGFVASKVVADQK
Protein #2 682 residues (38% resolved)

MPTFVEIDHVDRIFDLPNGGRYIALKNIELKIKQGEFVSLIGHSGCGKSTLLNIIAGLDRASIGGVTLEGREIREPSPDRMVVFQNYSLLPWLTVRENVALAVDEVYQGKSKGERRAIIEEHIDMVGLRLAANKRPSELSGGMKQRVAIARALATRPKLLLLDQPFGALDALTRGSLQEQLMKICNEHQITCVMVTHDVDEALLLSDRVVMLTNGPEAHIGQILEVPISRPRQRLEVVKHPSYYNLRNEIIYFLNQQKLAKKRQTQQASAPLGTAKAVIEIGFMPLTDSAPLIVAKEKGFFAKYGLDNVILNRANNWQAIATGVVTGKLDAAQMVAGMPIALTLGAGSQTPTPVINALNLSRNANAITFSKRLYNQGVRSLADLKAVIDSSPDQILTLGVVHSASMQNLILRYWLAAGGIDPDRDVSLTVIPPTQMVSQLKAGNIDGYCAGEPWNYQAVHDDLGFVAATALEIWSGQPKKVLGVREDWAQKYPETYLNLVKALIEACKYCDDLRNREEILEILCRPEYLDVNPAYVRSGFIDPYDRGDGTPPQQLTAYNQFYLNKTNYPNRTEILWMITQMARWGLTPFPKNWVEITERVCRTDIFGAAARDLGLLDIGEDDPIHLFDGKLFNPSEPIEYLKSLEIRRQIRIEEVFISSGDYKDHDGDYKDHDIDYKDDDDK

MPTFVEIDHVDRIFDLPNGGRYIALKNIELKIKQGEFVSLIGHSGCGKSTLLNIIAGLDRASIGGVTLEGREIREPSPDRMVVFQNYSLLPWLTVRENVALAVDEVYQGKSKGERRAIIEEHIDMVGLRLAANKRPSELSGGMKQRVAIARALATRPKLLLLDQPFGALDALTRGSLQEQLMKICNEHQITCVMVTHDVDEALLLSDRVVMLTNGPEAHIGQILEVPISRPRQRLEVVKHPSYYNLRNEIIYFLNQQKLAKKRQTQQASAPLGTAKAVIEIGFMPLTDSAPLIVAKEKGFFAKYGLDNVILNRANNWQAIATGVVTGKLDAAQMVAGMPIALTLGAGSQTPTPVINALNLSRNANAITFSKRLYNQGVRSLADLKAVIDSSPDQILTLGVVHSASMQNLILRYWLAAGGIDPDRDVSLTVIPPTQMVSQLKAGNIDGYCAGEPWNYQAVHDDLGFVAATALEIWSGQPKKVLGVREDWAQKYPETYLNLVKALIEACKYCDDLRNREEILEILCRPEYLDVNPAYVRSGFIDPYDRGDGTPPQQLTAYNQFYLNKTNYPNRTEILWMITQMARWGLTPFPKNWVEITERVCRTDIFGAAARDLGLLDIGEDDPIHLFDGKLFNPSEPIEYLKSLEIRRQIRIEEVFISSGDYKDHDGDYKDHDIDYKDDDDK
Protein #3 277 residues (92% resolved)

MQIINRNNQTNLKPQKTDNFLVVEGVSKIYPTPEGPYTVLDGIDLKVREGEFVCLIGHSGCGKSTLLNMISGFNTPSEGVVLLQDKPITEPGPDRMMVFQNYCLLPWLNVFENVYLAVDAVFPNKPQAEKRAIVREHLAMVGLTEAAEKKPSQISGGMKQRVAIARALSIRPQVLILDQPFGALDAITKEELQEELLQIWSDHQVTVLMITHDIDEALFLADRVVMMTNGPAAQIGEILDIPFDRPRNRRRIMEDPKYYDLRNYALDFLFNRFAHNE

MQIINRNNQTNLKPQKTDNFLVVEGVSKIYPTPEGPYTVLDGIDLKVREGEFVCLIGHSGCGKSTLLNMISGFNTPSEGVVLLQDKPITEPGPDRMMVFQNYCLLPWLNVFENVYLAVDAVFPNKPQAEKRAIVREHLAMVGLTEAAEKKPSQISGGMKQRVAIARALSIRPQVLILDQPFGALDAITKEELQEELLQIWSDHQVTVLMITHDIDEALFLADRVVMMTNGPAAQIGEILDIPFDRPRNRRRIMEDPKYYDLRNYALDFLFNRFAHNE
Non polymer #4 ATP

InChI=1S/C10H16N5O13P3/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20)/t4-,6-,7-,10-/m1/s1

c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO[P@@](=O)(O)O[P@](=O)(O)OP(=O)(O)O)O)O)N
Non polymer #5 MG

InChI=1S/Mg/q+2

[Mg+2]
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Reference